Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Digestion K823023 (t44)"

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|content=<div class="text">The miniprepped samples were digested to check for insert. It was seen that indeed tasA was inserted successfully into pSB1C3. The insert was stored as T2.</div>
 
|content=<div class="text">The miniprepped samples were digested to check for insert. It was seen that indeed tasA was inserted successfully into pSB1C3. The insert was stored as T2.</div>
  
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{{Team:Groningen/TEMPLATES/LOG
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|title=00:00, 16 July 2015 - 00:00, 16 July 2015
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|executor=Harm Ruesink
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|content=<div class="text">The salt promoter, tasA (T2) and the backbone were digested. For the digest the following combinations were made.</div>
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<div class="object data" id="tbl1">
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<div class="wrapper">
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<div class="header">
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<div class="field fw1">#</div>
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<div class="field fw3">DNA</div>
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<div class="field fw3">Restriction enzymes</div>
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</div>
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<div class="record">
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<div class="field fw1">1</div>
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<div class="field fw3">Psalt</div>
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<div class="field fw3">EcoRI + SpeI</div>
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</div>
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<div class="record">
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<div class="field fw1">2</div>
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<div class="field fw3">tasA (T2, 10-7-15)</div>
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<div class="field fw3">XbaI + PstI</div>
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</div>
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<div class="record">
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<div class="field fw1">3</div>
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<div class="field fw3">BBa_K823023</div>
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<div class="field fw3">EcoRI + PstI</div>
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</div>
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</div>
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<div class="caption">Digestion.</div>
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</div>
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 +
 +
<div class="object data" id="tbl1">
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<div class="wrapper">
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<div class="header">
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<div class="field fw1">#</div>
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<div class="field fw3">Component</div>
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<div class="field fw3">Amount</div>
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</div>
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<div class="record">
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<div class="field fw1">1</div>
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<div class="field fw3">DNA (Psalt)</div>
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<div class="field fw3">6 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
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<div class="field fw3">EcoRI</div>
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<div class="field fw3">1 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
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<div class="field fw3">SpeI</div>
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<div class="field fw3">1 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
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<div class="field fw3">10x buffer (2.1)</div>
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<div class="field fw3">2 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
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<div class="field fw3">\( \mathrm{H_2O}\)</div>
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<div class="field fw3">10 µL</div>
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</div>
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<div class="record">
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<div class="field fw1">2</div>
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<div class="field fw3">DNA (T2)</div>
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<div class="field fw3">3 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
 +
<div class="field fw3">XbaI</div>
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<div class="field fw3">1 µL</div>
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</div>
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<div class="record">
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<div class="field fw1"></div>
 +
<div class="field fw3">PstI</div>
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<div class="field fw3">1 µL</div>
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</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">10x buffer (2.1)</div>
 +
<div class="field fw3">2 µL</div>
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</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">\( \mathrm{H_2O}\)</div>
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<div class="field fw3">13 µL</div>
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</div>
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<div class="record">
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<div class="field fw1">3</div>
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<div class="field fw3">DNA (K823023)</div>
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<div class="field fw3">6 µL</div>
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</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">EcoRI</div>
 +
<div class="field fw3">1 µL</div>
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</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">PstI</div>
 +
<div class="field fw3">1 µL</div>
 +
</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">10x buffer (2.1)</div>
 +
<div class="field fw3">2 µL</div>
 +
</div>
 +
<div class="record">
 +
<div class="field fw1"></div>
 +
<div class="field fw3">\( \mathrm{H_2O}\)</div>
 +
<div class="field fw3">10 µL</div>
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</div>
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</div>
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<div class="caption">Components samples.</div>
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</div>
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<div class="text">The samples were incubated for one hour at 37 °C.</div>
 
}}
 
}}

Latest revision as of 12:42, 18 September 2015

00:00, 10 July 2015 - 00:00, 10 July 2015
The miniprepped samples were digested to check for insert. It was seen that indeed tasA was inserted successfully into pSB1C3. The insert was stored as T2.
Harm Ruesink
00:00, 16 July 2015 - 00:00, 16 July 2015
The salt promoter, tasA (T2) and the backbone were digested. For the digest the following combinations were made.
#
DNA
Restriction enzymes
1
Psalt
EcoRI + SpeI
2
tasA (T2, 10-7-15)
XbaI + PstI
3
BBa_K823023
EcoRI + PstI
Digestion.


#
Component
Amount
1
DNA (Psalt)
6 µL
EcoRI
1 µL
SpeI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
10 µL
2
DNA (T2)
3 µL
XbaI
1 µL
PstI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
13 µL
3
DNA (K823023)
6 µL
EcoRI
1 µL
PstI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
10 µL
Components samples.
The samples were incubated for one hour at 37 °C.
Harm Ruesink