Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Digestion K823023 (t44)"
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|content=<div class="text">The miniprepped samples were digested to check for insert. It was seen that indeed tasA was inserted successfully into pSB1C3. The insert was stored as T2.</div> | |content=<div class="text">The miniprepped samples were digested to check for insert. It was seen that indeed tasA was inserted successfully into pSB1C3. The insert was stored as T2.</div> | ||
+ | }} | ||
+ | {{Team:Groningen/TEMPLATES/LOG | ||
+ | |title=00:00, 16 July 2015 - 00:00, 16 July 2015 | ||
+ | |executor=Harm Ruesink | ||
+ | |content=<div class="text">The salt promoter, tasA (T2) and the backbone were digested. For the digest the following combinations were made.</div> | ||
+ | |||
+ | <div class="object data" id="tbl1"> | ||
+ | <div class="wrapper"> | ||
+ | <div class="header"> | ||
+ | <div class="field fw1">#</div> | ||
+ | <div class="field fw3">DNA</div> | ||
+ | <div class="field fw3">Restriction enzymes</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">1</div> | ||
+ | <div class="field fw3">Psalt</div> | ||
+ | <div class="field fw3">EcoRI + SpeI</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">2</div> | ||
+ | <div class="field fw3">tasA (T2, 10-7-15)</div> | ||
+ | <div class="field fw3">XbaI + PstI</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">3</div> | ||
+ | <div class="field fw3">BBa_K823023</div> | ||
+ | <div class="field fw3">EcoRI + PstI</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="caption">Digestion.</div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="object data" id="tbl1"> | ||
+ | <div class="wrapper"> | ||
+ | <div class="header"> | ||
+ | <div class="field fw1">#</div> | ||
+ | <div class="field fw3">Component</div> | ||
+ | <div class="field fw3">Amount</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">1</div> | ||
+ | <div class="field fw3">DNA (Psalt)</div> | ||
+ | <div class="field fw3">6 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">EcoRI</div> | ||
+ | <div class="field fw3">1 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">SpeI</div> | ||
+ | <div class="field fw3">1 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">10x buffer (2.1)</div> | ||
+ | <div class="field fw3">2 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">\( \mathrm{H_2O}\)</div> | ||
+ | <div class="field fw3">10 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">2</div> | ||
+ | <div class="field fw3">DNA (T2)</div> | ||
+ | <div class="field fw3">3 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">XbaI</div> | ||
+ | <div class="field fw3">1 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">PstI</div> | ||
+ | <div class="field fw3">1 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">10x buffer (2.1)</div> | ||
+ | <div class="field fw3">2 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">\( \mathrm{H_2O}\)</div> | ||
+ | <div class="field fw3">13 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1">3</div> | ||
+ | <div class="field fw3">DNA (K823023)</div> | ||
+ | <div class="field fw3">6 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">EcoRI</div> | ||
+ | <div class="field fw3">1 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">PstI</div> | ||
+ | <div class="field fw3">1 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">10x buffer (2.1)</div> | ||
+ | <div class="field fw3">2 µL</div> | ||
+ | </div> | ||
+ | <div class="record"> | ||
+ | <div class="field fw1"></div> | ||
+ | <div class="field fw3">\( \mathrm{H_2O}\)</div> | ||
+ | <div class="field fw3">10 µL</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="caption">Components samples.</div> | ||
+ | </div> | ||
+ | |||
+ | <div class="text">The samples were incubated for one hour at 37 °C.</div> | ||
}} | }} |
Latest revision as of 12:42, 18 September 2015
00:00, 10 July 2015 - 00:00, 10 July 2015
The miniprepped samples were digested to check for insert. It was seen that indeed tasA was inserted successfully into pSB1C3. The insert was stored as T2.
00:00, 16 July 2015 - 00:00, 16 July 2015
The salt promoter, tasA (T2) and the backbone were digested. For the digest the following combinations were made.
#
DNA
Restriction enzymes
1
Psalt
EcoRI + SpeI
2
tasA (T2, 10-7-15)
XbaI + PstI
3
BBa_K823023
EcoRI + PstI
#
Component
Amount
1
DNA (Psalt)
6 µL
EcoRI
1 µL
SpeI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
10 µL
2
DNA (T2)
3 µL
XbaI
1 µL
PstI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
13 µL
3
DNA (K823023)
6 µL
EcoRI
1 µL
PstI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
10 µL
The samples were incubated for one hour at 37 °C.