Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/tasA Digestion Psalt t51"

Latest revision as of 13:59, 18 September 2015

tasA Digestion Psalt (t51)

The salt promoter was digested to make ligation with tasA in the K823023 possible.

Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.

The digestion of the salt promoter was performed.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>

00:00, 23 July 2015 - 00:00, 23 July 2015

For the ligation of Psalt, tasA and BBa_K823023, first the salt promoter (Psalt) had to be digested using EcoRI and SpeI as restriction enzymes. tasA and BBA_K823023 had been digested before already.

DNA (Psalt)

10 µL

EcoRI

1 µL

SpeI

1 µL

10x buffer (2.1)

2 µL

\( \mathrm{H_2O}\)

6 µL

Components for digestion salt promoter.

The sample was digested for one hour at 37 °C.

Harm Ruesink

@@ Line 5: / Line 5: @@
 	|goal=<html>Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.</html>
 	|description=The salt promoter was digested to make ligation with tasA in the K823023 possible.
-	|conclusion=The ligation of the salt promoter was performed.
+	|conclusion=The digestion of the salt promoter was performed.
 }}