Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/tasA Sequencing t59"

Latest revision as of 14:54, 18 September 2015

tasA Sequencing (t59)

The tasA DNA was send for sequencing to check if the sequence was correct.

Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.

Sequencing showed that the tasA (T2) sample is correct.

00:00, 30 July 2015 - 00:00, 30 July 2015

The transformation of 29 July was checked. No colonies were visible on the LB+cm plate. T2 was send to Macrogen for sequencing.

1/20 primer (5 pmol/µL)

5 µL

plasmid DNA (T2)

5 µL

Amount of primers and DNA sent for sequencing.

prefix primer (forward)

17C4ZAD658

suffix primer (reverse)

17C4ZAD659

Sequencing codes Macrogen.

The results from Macrogen showed that T2 has the right sequence.

Harm Ruesink

@@ Line 4: / Line 4: @@
 	|protocolurl=None
 	|goal=<html>Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.</html>
-	|description=The tasA ligation products were send for sequencing to check if the ligation was successful.
+	|description=The tasA DNA was send for sequencing to check if the sequence was correct.
-	|conclusion=Sequencing showed that the ligation was successful.
+	|conclusion=Sequencing showed that the tasA (T2) sample is correct.
 }}