Difference between revisions of "Team:NJAU China/Parts"

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<div class="section">
 
<div class="section">
 
<h2>BBa_K1649000</h2>
 
<h2>BBa_K1649000</h2>
−
<p>red light sensor</p>
+
<p>Red light sensor</p>
 
<div class="first_down"><span>T</span>
 
<div class="first_down"><span>T</span>
 
<p>he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.</p>
 
<p>he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.</p>
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<img src="https://static.igem.org/mediawiki/2015/f/f0/NJAU_2015_teamparts1.png"/>
 
<img src="https://static.igem.org/mediawiki/2015/f/f0/NJAU_2015_teamparts1.png"/>
 
<h2>BBa_K1649001</h2>
 
<h2>BBa_K1649001</h2>
−
<p>red light sensor with a GFP reporter</p>
+
<p>Red light sensor with a GFP reporter</p>
 
<div class="first_down"><span class="first_down">G</span>
 
<div class="first_down"><span class="first_down">G</span>
 
<p>FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.</p>
 
<p>FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.</p>

Revision as of 15:57, 18 September 2015

BBa_K1649000

Red light sensor

T

he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.

BBa_K1649001

Red light sensor with a GFP reporter

G

FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.

BBa_K1649002

Red light sensor with TetR not gate and GFP reporter

A

s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.

BBa_K1649004

YF1-FixJ with terminator

T

he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.

BBa_K1649003

YF1 blue light sensor with GFP reporter

G

FP is used to report the function of blue light sensor.