Difference between revisions of "Team:GeorgiaTech/Results"
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<p>We are pleased to describe the results of our research endeavors! As a team, we:</p> | <p>We are pleased to describe the results of our research endeavors! As a team, we:</p> | ||
<ul><li>Successfully <a style="color:white"; href="https://2015.igem.org/Team:GeorgiaTech/Experiments#eppcr">mutated our starting proteins</a> using error-prone PCR.</li> | <ul><li>Successfully <a style="color:white"; href="https://2015.igem.org/Team:GeorgiaTech/Experiments#eppcr">mutated our starting proteins</a> using error-prone PCR.</li> | ||
| + | <li>Achieved an error rate of about 0.2% - 0.5%.</li> | ||
<li>Generated a diverse library of protein mutants to be used for future experiments.</li> | <li>Generated a diverse library of protein mutants to be used for future experiments.</li> | ||
<li>Submitted mutated Plastocyanin gene sequences to the iGEM Parts Registry in <a style="color:white"; href="https://2015.igem.org/Team:GeorgiaTech/Parts">standard BioBrick format.</a></li></ul> | <li>Submitted mutated Plastocyanin gene sequences to the iGEM Parts Registry in <a style="color:white"; href="https://2015.igem.org/Team:GeorgiaTech/Parts">standard BioBrick format.</a></li></ul> | ||
Revision as of 16:54, 18 September 2015
Experiment Results
We are pleased to describe the results of our research endeavors! As a team, we:
- Successfully mutated our starting proteins using error-prone PCR.
- Achieved an error rate of about 0.2% - 0.5%.
- Generated a diverse library of protein mutants to be used for future experiments.
- Submitted mutated Plastocyanin gene sequences to the iGEM Parts Registry in standard BioBrick format.
Future Directions
As a team, we have several future plans for our project:
- Perform DNA shuffling on our mutated proteins in order to enhance the diversity of our library.
- Screen our mutated proteins for activity and function using phage display.
- Use these techniques to evolve a “clickase” that will successfully acquire copper for the CuACC reaction in vivo.
- This “clickase” will be a ligand that can accelerate the CuACC reaction in vivo.
Why DNA Shuffling?
- Increases diversity of genes by combining different segments of homologous genes
- Forms unnatural chimeric genes with possible new activities
- Starting with naturally occurring homologous sequences allows for combination of the most useful mutations from individual genes.
Achieving our future goals would have widespread applications that could greatly advance drug delivery and molecular tagging, and the research we conducted this summer has laid a solid foundation for our future plans.
Beyond the Bench
Aside from our research achievements, we also completed several projects beyond the bench. As a team, we:
- Collaborated with Lambert High School to:
- Debug their construct
- Critique their presentations
- Created an informational Georgia Tech iGEM video series:
- One central source for lab techniques and background
- Will better equip future iGEM teams for successful research
- Distributed iGEM informational pamphlets to:
- Spread the word about iGEM and synthetic biology
- Teach college students about our project and achievements
Here you can describe the results of your project and your future plans.
What should this page contain?
- Clearly and objectively describe the results of your work.
- Future plans for the project
- Considerations for replicating the experiments
Project Achievements
You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.