Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"

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<h1>BioBricks</h1>
 
<h1>BioBricks</h1>
  
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<h2>1. BBa_K1603000: STE2MAM2</h2>
+
<h2>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h2>
 
<p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.</p>
 
<p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.</p>
−
<p>http://parts.igem.org/Part:BBa_K1603000</p>
+
 
−
<h2>2. BBa_K1603001: pTEF1-pSUC2</h2>
+
<h2>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h2>
 
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
 
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
−
<p>http://parts.igem.org/Part:BBa_K1603001</p>
+
 
−
<h2>3. BBa_K1603002: pTPI1</h2>
+
<h2>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h2>
 
<p>Promoter to TPI1.</p>
 
<p>Promoter to TPI1.</p>
−
<p>http://parts.igem.org/Part:BBa_K1603002</p>
+
 
−
<h2>4. BBa_K1603003: RecA</h2>
+
<h2>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h2>
 
<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.  
 
<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.  
 
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
−
<p>http://parts.igem.org/Part:BBa_K1603003</p>
+
 
−
<h2>5. BBa_K1603004: SSB</h2>
+
<h2>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h2>
 
<p>Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
<p>Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
−
<p>http://parts.igem.org/Part:BBa_K1603004</p>
 

Revision as of 16:55, 18 September 2015



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BioBricks

1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2

Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.

2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1

Promoter to TPI1.

4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA

Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.

5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB

Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.