Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"
Line 325: | Line 325: | ||
<h1>BioBricks</h1> | <h1>BioBricks</h1> | ||
− | <h2>1. BBa_K1603000: STE2MAM2</h2> | + | <h2>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h2> |
<p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.</p> | <p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.</p> | ||
− | < | + | |
− | + | <h2>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h2> | |
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p> | <p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p> | ||
− | < | + | |
− | + | <h2>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h2> | |
<p>Promoter to TPI1.</p> | <p>Promoter to TPI1.</p> | ||
− | < | + | |
− | + | <h2>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h2> | |
<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. | <p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. | ||
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> | Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> | ||
− | < | + | |
− | + | <h2>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h2> | |
<p>Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> | <p>Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> | ||
− |
Revision as of 16:55, 18 September 2015
Contents
- 1 BioBricks
- 1.1 1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2
- 1.2 2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2
- 1.3 3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1
- 1.4 4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA
- 1.5 5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB
BioBricks
1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2
Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.
2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2
The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.
3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1
Promoter to TPI1.
4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA
Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.
5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB
Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.