Difference between revisions of "Team:Warwick/Description"

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<ul> <li> The JO4450 gene from the registry was cloned into three different plasmids (pSB1K3, pSB3K3, and pSB4K4) each with a different copy number. </li>
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<ul> <li> The J04450 gene from the registry was cloned into three different plasmids (pSB1K3, pSB3K3, and pSB4K4) each with a different copy number. </li>
 
<li>Each plasmid was transformed electrocompetent into MG1655-Z1</li>
 
<li>Each plasmid was transformed electrocompetent into MG1655-Z1</li>
 
<li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li>
 
<li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li>
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<p>The results for our characterisation can be found on the JO4450 part page in the registry.</p>
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<p>The results for our characterisation can be found on the<a href="http://parts.igem.org/Part:BBa_J04450">J04450 part page in the registry</a>.</p>
 
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Revision as of 17:56, 18 September 2015

Warwick iGEM 2015