Difference between revisions of "NJU-China-notebook.html"
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Exosomes (100 μg) loaded with MOR siRNAs were incubated with Neuro2A cells (10<sup>6</sup> cells). After 24 h incubation, the recipient cells were collected for total RNA extraction and subsequent quantitative RT-PCR analysis of MOR siRNA and MOR mRNA, and for total protein isolation and subsequent western blotting analysis of MOR protein. | Exosomes (100 μg) loaded with MOR siRNAs were incubated with Neuro2A cells (10<sup>6</sup> cells). After 24 h incubation, the recipient cells were collected for total RNA extraction and subsequent quantitative RT-PCR analysis of MOR siRNA and MOR mRNA, and for total protein isolation and subsequent western blotting analysis of MOR protein. | ||
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In CPP test, researchers place an animal in a distinctively designed chamber and inject or infuse it with the substance being studied (e.g., morphine in this study). Once trained to associate the chamber with the test agent, the animal is placed in an anteroom connecting a neutral chamber and the drug-associated chamber. The animal indicates its preferred chamber by spending more time there; its choice reveals the animal’s preference for or aversion to the test substance. </br> | In CPP test, researchers place an animal in a distinctively designed chamber and inject or infuse it with the substance being studied (e.g., morphine in this study). Once trained to associate the chamber with the test agent, the animal is placed in an anteroom connecting a neutral chamber and the drug-associated chamber. The animal indicates its preferred chamber by spending more time there; its choice reveals the animal’s preference for or aversion to the test substance. </br> | ||
Here, C57BL/6J mice (n=6 for each group) were purchased from the Model Animal Research Centre (MARC) of Nanjing University (Nanjing, China). The animals received humane care according to the guidelines prepared by the National Institutes of Health Guide for the Care and Use of Laboratory Animals. A two-chamber CPP apparatus (Yishu Software Technology Co. Ltd., Shanghai, China) was used in this study. The two chambers were identical in size but differed in color and floor texture. The two distinct chambers were linked by a smaller intermediate compartment with a shutter on each side that allowed for control of access to either side of the chamber. One chamber had white walls with a barred floor and was illuminated, while the other had black walls with a dotted floor and was not illuminated. </br> | Here, C57BL/6J mice (n=6 for each group) were purchased from the Model Animal Research Centre (MARC) of Nanjing University (Nanjing, China). The animals received humane care according to the guidelines prepared by the National Institutes of Health Guide for the Care and Use of Laboratory Animals. A two-chamber CPP apparatus (Yishu Software Technology Co. Ltd., Shanghai, China) was used in this study. The two chambers were identical in size but differed in color and floor texture. The two distinct chambers were linked by a smaller intermediate compartment with a shutter on each side that allowed for control of access to either side of the chamber. One chamber had white walls with a barred floor and was illuminated, while the other had black walls with a dotted floor and was not illuminated. </br> |
Revision as of 18:22, 18 September 2015
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Notebook
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