Difference between revisions of "Team:UFSCar-Brasil/protocols.html"
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<h3 class="ui header" id="overview">Below are presented all used protocols along wet lab. To see more, please dowload them!</h3> | <h3 class="ui header" id="overview">Below are presented all used protocols along wet lab. To see more, please dowload them!</h3> | ||
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<a class="item" href="https://static.igem.org/mediawiki/2015/7/74/UFSCar-Brasil_Zinc_purification.pdf">Batch purification of Zinc Proteins</a> | <a class="item" href="https://static.igem.org/mediawiki/2015/7/74/UFSCar-Brasil_Zinc_purification.pdf">Batch purification of Zinc Proteins</a> | ||
<a class="item" href="https://static.igem.org/mediawiki/2015/3/3b/UFSCar-Brasil_protocol02.pdf">Procedures to turn a Ni-NTA resin into Zn-NTA resin</a> | <a class="item" href="https://static.igem.org/mediawiki/2015/3/3b/UFSCar-Brasil_protocol02.pdf">Procedures to turn a Ni-NTA resin into Zn-NTA resin</a> | ||
Latest revision as of 19:26, 18 September 2015
Protocols
We do, what we do
Below are presented all used protocols along wet lab. To see more, please dowload them!
Batch purification of Zinc Proteins
Procedures to turn a Ni-NTA resin into Zn-NTA resin
Protocol to measure activities of SmtA and Zur proteins
Protocol to production of [6xHis]-GFP
Protocol to Imobilized Metal Affinity Chromatography (IMAC) protein purification
Standard curve of GFP fluorescence
Protocol to production of wtGFP under different promoters
Protocol to promoter activity test under carbon starving
Protocol to cold shock effect in GFP expression coordinated by uspA and J23101
Protocol of Agarose Gel
Protocol of DNA purification
Protocol of Polymerase Chain Reaction (PCR)
Protocol of SDS-PAGE
Protocol of Cells Transformation
Culture Media
Plasmolysis Protocol
Protocol of Serial Dilutions Platting
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