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| | <h3>Biological Risks</h3> | | <h3>Biological Risks</h3> |
| − | <p>Below is a table that summarizes the vectors and strains that were used during the course of our research:</p> | + | <p>Below is a table that summarizes the vectors and strains that were used during the course of our rese |
| − | <table>
| + | |
| − | <tr>
| + | |
| − | <th>Species Name</th>
| + | |
| − | <th>Risk Group</th>
| + | |
| − | <th>Risk Group Source</th>
| + | |
| − | <th>Pathogenic?</th>
| + | |
| − | <th>Part Number/Name</th>
| + | |
| − | <th>Company</th>
| + | |
| − | <th>How will you use it?</th>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td><i>E.Coli</i> (DH5α<sup>TM</sup>)</td>
| + | |
| − | <td>0</td>
| + | |
| − | <td>MSDS</td>
| + | |
| − | <td>no</td>
| + | |
| − | <td><i>E. Coli</i> competent cell line</td>
| + | |
| − | <td>Life Technologies</td>
| + | |
| − | <td>Cloning</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td><i>E.Coli</i> (BL21)</td>
| + | |
| − | <td>0</td>
| + | |
| − | <td>MSDS</td>
| + | |
| − | <td>no</td>
| + | |
| − | <td><i>E. Coli</i> competent cell line</td>
| + | |
| − | <td>Life Technolgies</td>
| + | |
| − | <td>Expression testing</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>TOPO cloning vector</td>
| + | |
| − | <td>0</td>
| + | |
| − | <td>MSDS</td>
| + | |
| − | <td>no</td>
| + | |
| − | <td>TOPO cloning vector</td>
| + | |
| − | <td>Life Technologies</td>
| + | |
| − | <td>Cloning</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>pET23-b expression vector</td>
| + | |
| − | <td>0</td>
| + | |
| − | <td>MSDS</td>
| + | |
| − | <td>no</td>
| + | |
| − | <td>pET23-b expression vector</td>
| + | |
| − | <td>Life Technologies</td>
| + | |
| − | <td>Expression testing</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>pUC57 cloning vector</td>
| + | |
| − | <td>0</td>
| + | |
| − | <td>MSDS</td>
| + | |
| − | <td>no</td>
| + | |
| − | <td>pUC57 cloning vector</td>
| + | |
| − | <td>GeneScript</td>
| + | |
| − | <td></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <h3>Future Risks</h3>
| + | |
| − | <p>The human body is a very complex system. Even though the CuAAC reaction has very specific substrates and conditions, the clickase enzyme could have unforeseen reactions with biological molecules, or it could compete with already-existing copper-binding proteins. Future work would include in-vivo studies.</p>
| + | |
| − | | + | |
| − | <h3>Safety Precautions</h3>
| + | |
| − | <p>At all times when working in the laboratory, our team members wore nitrile gloves, safety goggles, closed-toed shoes, protective lab coats, and long pants (PPE). Gloves were disposed of properly and hands were washed immediately after exiting the lab space. Experienced mentors (graduate researchers) were always around to assist in any situation that was in question.</p>
| + | |
| − | <p>Any waste that was to come into contact with a biologic was disposed of in its proper biohazard container and autoclaved. Also, any broken glass was disposed of in its proper sharps container.</p>
| + | |
| − | | + | |
| − | <h3>Researcher Safety</h3>
| + | |
| − | <p>All team members completed RCR (Responsible Conduct of Research) training through the CITI program, basic biohazard safety, fire safety, RTK (Right to Know) safety training, hazardous waste, recombinant DNA training, and blood-borne pathogen training.</h3>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class = "band" style = "background-color:#ebb211">
| + | |
| − | <p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
| + | |
| − | | + | |
| − | <p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
| + | |
| − | | + | |
| − | | + | |
| − | <h4>Safe Project Design</h4>
| + | |
| − | | + | |
| − | <p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
| + | |
| − | | + | |
| − | <ul>
| + | |
| − | <li>Choosing a non-pathogenic chassis</li>
| + | |
| − | <li>Choosing parts that will not harm humans / animals / plants</li>
| + | |
| − | <li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
| + | |
| − | <li>Including an "induced lethality" or "kill-switch" device</li>
| + | |
| − | </ul>
| + | |
| − | | + | |
| − | <h4>Safe Lab Work</h4>
| + | |
| − | | + | |
| − | <p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
| + | |
| − | | + | |
| − | <h4>Safe Shipment</h4>
| + | |
| − | | + | |
| − | <p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </html>
| + | |
