Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Colony PCR (t102)"

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Revision as of 00:33, 19 September 2015

00:00, 18 August 2015 - 00:00, 18 August 2015
Plates were checked for colonies. Plate with salt promoter in pSB1C3 contained colonies. 4 colonies were checked with colony PCR, therefore VF and VF2 primers were used.
#
Temperature
Time
Step
1
98 °C
5:00
Initial denaturation
2
98 °C
0:10
Denaturation
75 °C
0:30
Annealing
72 °C
1:30
Extension
repeat 35x
3
72 °C
5:00
PCR Thermocycle
Each transformation had at least one correct insert according to the PCR. This colony was grown in LB with corresponding antibiotics overnight at 37 °C.
Marieke Mulder