Difference between revisions of "Team:NTU-Singapore/Design"

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<h5>Design</h5>
 
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<p class="subtitle explain">As mentioned in the concept, we will be using the Arduino board as the voltage reader, hence, the programming for the Arduino board has to be done so that it can be used along with a LCD display screen.
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<p class="subtitle explain">As mentioned in the concept, we will be using the Arduino board as the voltage reader, hence, the programming for the Arduino board has to be done so that it can be used along with a LCD display screen.  
 
 
 
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<h5>Testing It Out</h5>
 
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<p class="subtitle explain">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Fusce mollis sem porta, tempus nunc et, rutrum tortor. Donec eleifend nunc non mauris hendrerit, eu ornare ipsum auctor. Nunc pellentesque dui eu sagittis porttitor. Vivamus posuere volutpat magna sed ultrices. Phasellus egestas lacinia massa. Praesent gravida, eros a laoreet ultricies, arcu ligula tincidunt nisi, sed pretium ante nisi a lectus. Integer nec cursus massa, vel commodo justo. Nulla maximus est mi, vitae rhoncus turpis venenatis ac. Interdum et malesuada fames ac ante ipsum primis in faucibus. Nulla aliquet aliquet lectus et vestibulum. Nunc vulputate ligula in feugiat euismod. Suspendisse potenti.
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<p class="subtitle explain"> We wanted to test whether our cartridge would work. Hence, we culture Shewanella oneidensis MR1 containing the lactate permease and lactate dehydrogenase operon and used it for our cartridge. The measurement was made both with the Arduino as well as a multimeter as we would like to have a comparison of any deviations from the multimeter. However, no significant deviations was  detected as the values reflected in the Arduino closely matches the value in the multimeter.
 
 
 
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<h5>Results</h5>
 
<h5>Results</h5>
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<p class="subtitle explain">From the data, we can see that there is an initial rise of the voltage to 679mV and starts to decline after 42mins. This suggests that our cartridge works and further development on the cartridge can be carried out. More specifically, the LacR protein can be conjugated into this strain as the promoter used for our operon is pLac. Hence, the induction of the sensor should be further investigated. However, this is not done in our project due to the time constraints.  
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<img class="goleft" src="https://static.igem.org/mediawiki/2015/1/18/Shezensor_result.png" width="720px" height="550px">
 
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Revision as of 02:09, 19 September 2015

NTU SG iGEM 2015




Shezensor 1.0

The Concept

As MFCs have the potential to be used as a biosensor which is able to detect various chemicals and produce an electrical output. The ability to convert chemical to electrical signals gives MFCs an advantage over biosensors which rely on chemical outputs, i.e. fluorescence proteins as the electrical output allows on-field monitoring, automated data collection as well as WIFI communication.
For our project, our idea to make a portable device which allows users to test the cleanliness of water samples. Our device has two important components, the reader and the sensor. The reader is an arduino-controlled voltmeter and has a 16x2 LCD screen which provides a voltage reading to the user. While the sensor is a disposable cartridge which houses the MFC. Liquid samples can be loaded into the sensors and plugged into the reader to check if the voltage reading. After designing the cartridge, we carried out a proof-of-concept experiment to test the feasibility of our concept by first testing whether our disposable cartridge works.


Design

As mentioned in the concept, we will be using the Arduino board as the voltage reader, hence, the programming for the Arduino board has to be done so that it can be used along with a LCD display screen.

The Blueprint.

The Catridge.

The Reader.


Testing It Out

We wanted to test whether our cartridge would work. Hence, we culture Shewanella oneidensis MR1 containing the lactate permease and lactate dehydrogenase operon and used it for our cartridge. The measurement was made both with the Arduino as well as a multimeter as we would like to have a comparison of any deviations from the multimeter. However, no significant deviations was detected as the values reflected in the Arduino closely matches the value in the multimeter.

Kean Hean making final adjustments to the reader. :)

Walla!! Shezensor showing initial readings. On the right side is the cartridge port.

Shezonsor and friend!! :)