Difference between revisions of "Team:Aachen/Notebook/Documentation/Glycogen Characterization"
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* cryo cultures of #XULU# in BL21 Gold ΔglgP: #NKKA# #E4QF# #YNAZ# #R8LW# #B8FD# | * cryo cultures of #XULU# in BL21 Gold ΔglgP: #NKKA# #E4QF# #YNAZ# #R8LW# #B8FD# | ||
− | * Dinitrosalicylic acid staining (OD of WT, and ΔglgP were adjusted) | + | * Dinitrosalicylic acid staining (OD of WT, ΔglgX and ΔglgP were adjusted) |
* 1:100, 1:50, 1:25, 1:12.5, 1:6.25, 1:3.125, and 1:1.5625 dilutions of WT, ΔglgP, and ΔglgX were prepared; 50 µL sample volume. The same volume of Dinitrosalicylic acid was added; total volume was 100 µL. | * 1:100, 1:50, 1:25, 1:12.5, 1:6.25, 1:3.125, and 1:1.5625 dilutions of WT, ΔglgP, and ΔglgX were prepared; 50 µL sample volume. The same volume of Dinitrosalicylic acid was added; total volume was 100 µL. | ||
* blank: 1:1 dilution of dinitrosalicylic acid in water. | * blank: 1:1 dilution of dinitrosalicylic acid in water. |
Revision as of 03:18, 19 September 2015