Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Restriction ferritin (fr3)"
| Line 30: | Line 30: | ||
<div class="field fw3">EcoRI</div> | <div class="field fw3">EcoRI</div> | ||
<div class="field fw7">1 µL</div> | <div class="field fw7">1 µL</div> | ||
| − | + | </div> | |
<div class="record"> | <div class="record"> | ||
<div class="field fw1"></div> | <div class="field fw1"></div> | ||
<div class="field fw3">\( \mathrm{H_2O}\)</div> | <div class="field fw3">\( \mathrm{H_2O}\)</div> | ||
<div class="field fw7">6 µL</div> | <div class="field fw7">6 µL</div> | ||
| + | </div> | ||
</div> | </div> | ||
<div class="caption">Components for digestion.</div> | <div class="caption">Components for digestion.</div> | ||
</div> | </div> | ||
}} | }} | ||
Revision as of 21:45, 21 November 2015
16:00, 31 August 2015 - 18:30, 10 August 2015
Digest ferritin in order to ligate them. Samples were digested for 2 hours at 37 °C.
#
Component
Amount
1
ferritin
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.