Difference between revisions of "Team:Paris Saclay/Notebook/July/7"
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Revision as of 10:22, 15 July 2015
Contents
Tuesday 7th July
Back in Lab
Verification of our plasmid
by Coralie, Johan, Seong Koo, Audrey, Pauline
Biobricks verification:
- K115017
- C0040
- I13602
- J23101
- J23117
- J23106
- I13504
- K1493504
Extraction with Kit nucleospin
Digestion by NotI :
Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI
We use 18uL of this solution with 2uL plasmid solution
Digestion is process during 1h30 at 37°C
Electrophoresis
by Johan, Audrey, Pauline
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V
New culture
by Pauline, Audrey
New liquid cultures of:
- K1493504
2x 5ml LB + 10μl Chloramphenicol + 1 bacteria colonies. We incubate cultures at 37°C.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline