Difference between revisions of "Team:Reading/Notebook"

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Lab Book

Dates are in the UK format DD/MM/YY

29/06/15

Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of .6803 WT.

Cultured 5 streak plates of .6803 on solid BG-11

All were placed on the windowsill in our lab in natural light

02/07/15

Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of .6803 WT and 100ml of BG-11. They were exposed to 24hr light by the overhead lights in the lab.

07/07/15

Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4.

10/07/15

2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols.

13/07/15

Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x10⁸

OD₇₅₀ = 0.028

So

0.028x1.6x10⁸ = 4.48x10⁶ cells per ml

14/07/15

The lamp safety testing was completed so our flasks were placed under the white light bulb

What should this page have?

Chronological notes of what your team is doing.
Brief descriptions of daily important events.
Pictures of your progress.
Mention who participated in what task.

Date	OD₇₅₀	Estimated Cells per ml
7/7	0.000	0

@@ Line 26: / Line 26: @@
 </tr>
 <p> <b> 10/07/15 </b> </p>
-<p> 2 Litres of water collected from Reading University Lake for SODIS, method outlines in protocols. </p>
+<p> 2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols. </p>
 <p> <b> 13/07/15 </b> </p>
 <p> Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x10<sup>8</sup> <li> OD<sub>750</sub> = 0.028 </li> So <li> 0.028x1.6x10<sup>8</sup> = 4.48x10<sup>6</sup> cells per ml </li> </p>