Difference between revisions of "Team:Reading/Experiments"

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<h2>Protocols</h2>
 
<h2>Protocols</h2>
  
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<p>This page is an account of all protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.</p>
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<p>Note: This page is an account of all protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.</p>
  
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<p>  
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<p><h4>Growth of <i>Synechocystis sp. PCC 6803</i></h4>
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<ol>
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<li>Thaw stock of 6803 on ice</li>
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<li>100ml of BG-11 medium added to sterile conical flask aseptically</li>
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<li>Add 10µl or 5 loopfuls of 6803 to flask</li>
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<li>Incubate at ~30°C and on shaker at ~50rpm, under constant illumination</li>
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<li>Check growth each day by measuring OD<sub>750</sub></li>
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</ol></p>
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<h5>What should this page contain?</h5>
 
<h5>What should this page contain?</h5>

Revision as of 16:06, 18 July 2015



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Protocols

Note: This page is an account of all protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.

Growth of Synechocystis sp. PCC 6803

  1. Thaw stock of 6803 on ice
  2. 100ml of BG-11 medium added to sterile conical flask aseptically
  3. Add 10µl or 5 loopfuls of 6803 to flask
  4. Incubate at ~30°C and on shaker at ~50rpm, under constant illumination
  5. Check growth each day by measuring OD750

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

References