Difference between revisions of "Team:Reading/Experiments"

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<h2>Protocols</h2>
 
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<p>Note: This page is an account of all protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.</p>
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<p>Note: This section is an account of all laboratory protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.</p>
  
 
<p><h5>Growth of <i>Synechocystis sp. PCC 6803</i></h5>
 
<p><h5>Growth of <i>Synechocystis sp. PCC 6803</i></h5>
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<h2>Experiments</h2>
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<p> This section documents the designs and protocols for experiments we carried out during our project </p>
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<p><h5>Ferricyanide assay to measure electron output of 6803: Protocol</h5></p>
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<p><h5>Public perception and awareness study: Methods and Questionaire</h5></p>
 
<h5>What should this page contain?</h5>
 
<h5>What should this page contain?</h5>
 
<ul>
 
<ul>

Revision as of 17:21, 18 July 2015



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Protocols

Note: This section is an account of all laboratory protocols used by the team this year. The references at the bottom of the page provide the sources for the protocols, however we have modified several of them to our needs.

Growth of Synechocystis sp. PCC 6803
  1. Thaw stock of 6803 on ice
  2. 100ml of BG-11 medium added to sterile conical flask aseptically
  3. Add 10µl or 5 loopfuls of 6803 to flask
  4. Incubate at ~30°C and on shaker at ~50rpm, under constant illumination
  5. Check growth each day by measuring OD750

Taking optical density of culture to measure growth
We measured the OD750 to match that used in research papers on Synechocystis1.
  1. Set spectrophotometer to measure at OD750
  2. Blank with 1ml of BG-11
  3. Take OD750 of 1ml of culture
  4. If OD750 over 1 a.u, dilute 250µl of culture in 750µl of BG-11 (1/4 dilution)
To calculate cell density, we used the equation, 1 a.u = 1.6x108 cells per ml of culture2.

Experiments

This section documents the designs and protocols for experiments we carried out during our project

Ferricyanide assay to measure electron output of 6803: Protocol

Public perception and awareness study: Methods and Questionaire

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

References

  1. Bradley, R. W., Bombelli, P., Lea-Smith, D. J. & Howe, C. J. Terminal oxidase mutants of the cyanobacterium Synechocystis sp. PCC 6803 show increased electrogenic activity in biological photo-voltaic systems. Phys. Chem. Chem. Phys. PCCP 15, 13611–13618 (2013).
  2. Pojidaeva E, Zichenko V, Shestakov SV, Sokolenko A (2004) Involvement of the SppA1 peptidase in acclimation to saturating light intensities in Synechocystis sp. strain PCC 6803. J Bacteriol 186: 3991–3999.