Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
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===Quantification=== | ===Quantification=== | ||
Revision as of 15:01, 4 August 2015
Contents
Friday 27th July
Lab Work
New culture
by Seong Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Ligation
by Pauline and Coralie
K1707008
- 2µL Tp 10x
- 1µL Ligase
- 11µL BBa_K1707003
- 6µL BBa_J23101
K1707010
- 1µL Tp 10x
- 1µL Ligase
- 12µL BBa_K1399005
- 10µL BBa_B0015
- 1µL H20
K1707009
- 2µL Tp 10x
- 2µL Ligase
- 10µL BBa_S03518
- 6µL BBa_R0051
K1707004
- 3µL Tp 10x
- 2µL Ligase
- 8µL BBa_K1707003
- 7µL BBa_R0051
Transformation
by Pauline and Coralie
Biobricks:
- BBa_K1707008
- BBa_K1707010
- BBa_K1707009
- BBa_K1707004
Quantification
Biobricks:
- BBa_K1707003
- BBa_K1399005
- BBa_B0015
- BBa_J23101
Agarose gel 1% Migration 110V
Transformation
by Seong-Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Soil experiment
by Seong Koo
Plates observation and count of CFU:
- Strain 1696:
- Dilution 1: 424 CFU
- Dilution 0.1: 75 CFU
- Dilution 0.01: 1 CFU
- Strain 1693:
- Dilution 1: 524 CFU
- Dilution 0.1: 35 CFU
- Dilution 0.01: 0 CFU
- Strain 1320:
- Dilution 1: 111 CFU
- Dilution 0.1: 13 CFU
- Dilution 0.01: 0 CFU
We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C
Member present:
- Instructors: Alice
- Students: Coralie, Audrey, Pauline, Johan and Seong-Koo
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.