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• Team:British Columbia/Growing2
  <strong>PCR of inserts for cloning - Phusion DNA polymerase</strong> <p>To amplify an insert for cloning, using high-fidelity phusion polymerase.</p>
  4 KB (433 words) - 21:43, 31 August 2015
• Team:UCLA/Notebook/Honeybee Silk/18 July 2015
  ='''Picking colonies and Colony PCR of 7/17 Transformation'''= ===Colony PCR Reaction===
  11 KB (1,433 words) - 20:16, 20 July 2015
• Team:UCLA/Notebook/Honeybee Silk/8 July 2015
  ='''PCR of Honeybee G-block and Purification'''= ...ion and purification was too low (20.12ng/uL), today I will be redoing the PCR, digestion, and purification to obtain a higher yield.
  11 KB (1,323 words) - 20:14, 15 July 2015
• Team:UCLA/Notebook/Honeybee Silk/14 July 2015
  ='''PCR of Lac promoter/Silk/Spycatcher'''= !Q5 High Fidelity DNA Polymerase
  11 KB (1,451 words) - 21:47, 24 August 2015
• Team:Paris Bettencourt/Protocols/PCR
  <td><b>PCR protocol (Phusion)</b><br /> <li>25µL of <i>Life Technologies</i> Phusion High-Fidelity PCR Master Mix with HF Buffer</li>
  1 KB (176 words) - 16:05, 1 September 2015
• Team:UC Davis/Safety
  0.5 uL of Phusion High-Fidelity DNA Polymerase (Thermofisher) - added last<br> The PCR products of both the insert and the linearized plasmid were then combined i
  25 KB (3,709 words) - 05:04, 21 December 2015
• InterLab Study.html
  ...d Suffix-R Primers. After that, pcr products were cleaned up with QIAquick PCR Purification Kit. PCR- reaction
  4 KB (520 words) - 01:28, 9 September 2015
• Team:IISER Pune/Experiments
  ...as subjected to reverse transcription PCR (RT-PCR) using Transcriptor High Fidelity cDNA Synthesis Kit (Roche,Penzberg, Germany) to generate cDNA.</br> The PCR segments were digested with specific enzymes and ligated with linearized pc
  9 KB (1,271 words) - 02:41, 19 September 2015
• Team:UCLA/Notebook/Recombinant Expression
  <li>PCR'd off template</li> <p>PCR Reaction:</p>
  9 KB (1,187 words) - 07:18, 14 July 2015
• Team:UCLA/Notebook/Honeybee Silk/26 May 2015
  ='''PCR of Honeybee G-Block'''= *Diagnostic PCR, will be running a temperature gradient
  14 KB (1,912 words) - 20:39, 21 July 2015
• Team:UiOslo Norway/Experiments/PCR phusion
  <h1>PCR with Phusion-HF DNA Polymerase (NEB):</h1> Using the guidelines of the provided Phusion® High-Fidelity DNA Polymerase (M0530)
  3 KB (345 words) - 12:09, 18 September 2015
• Team:ETH Zurich/Materials
  <li>Product: Q5® High-Fidelity 2X Master Mix </li> <a href="https://www.neb.com/products/m0492-q5-high-fidelity-2x-master-mix"><p>for further information click here</p></a>
  27 KB (4,268 words) - 23:53, 18 September 2015
• Team:UChile-OpenBio/Atributions
  ...yle="color:#660066">Sigma Aldrich</span></b> is a leading Life Science and High Technology company focused on enhancing human health and safety, manufactur ...isms, amplify them and then produce the assemblies by overlap extension by PCR. Thanks to gBlocks we could save all this work in laboratory that would req
  41 KB (5,516 words) - 17:07, 14 November 2015
• Team:UChile-OpenBio/Attributions
  ...yle="color:#660066">Sigma Aldrich</span></b> is a leading Life Science and High Technology company focused on enhancing human health and safety, manufactur ...isms, amplify them and then produce the assemblies by overlap extension by PCR. Thanks to gBlocks we could save all this work in laboratory that would req
  41 KB (5,516 words) - 17:58, 14 November 2015
• Team:Pasteur Paris/Week 14
  ...align:left">PCR amplification of BBa_K936011 and BBa_K936023 using Q5 High fidelity Master Mix.</li>
  9 KB (1,123 words) - 02:18, 19 September 2015
• Team:Aachen/Lab/Methanol/Polycistronic Expression Plasmid
  ...es compatible with the RDP standard, they were amplified via high fidelity PCR with the primers that we designed previously. The templates were the coding
  5 KB (829 words) - 20:46, 18 September 2015