Troubleshooting/Restriction

Troubleshooting

• Introduction
• General Tips and Tricks
1. Transformation
2. Ligation
• iGEM Protocols
• Answers
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Ligation Troubleshooting

Now that you know you transformation efficiency and it's above 1 x 10⁸ CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with restriction digests.

No colonies

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