Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with kanomycin and picked colonies of PET24a in order to miniprep and generate more plasmid.
We are growing the starter culture for the silk protein up to an OD of 0.8.
Because we started the incubation at 3pm, and we dont want to overgrow, we will put the cells in the 30 C shaker until 10 pm, at which point we will transfer to the 37 degree shaker.
We will transfer the starter culture to a larger volume tomorrow morning 5/18