Team:Toulouse/Experiments

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Experiments & Protocols

Transformation Protocol: RbCl method

• - Media and solution
• - Competent Cells
• - Miniprep
• - Transformation

Cloning

• - Digestion
• - Parts have the same antibiotic resistance
• - Parts have different antibiotic resistances
• - Ligation
• - Transformation

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YETM 500mL	TFB1 200mL	TFB2 200mL
2.5g Yeast Extract 10g Tryptone 5g MgSO4.7H2O Adjust pH to 7.5 with KOH For Plates: add 7.5g of Agar	0.59g KOAc 2.42g RbCl 0.29g CaCl2.2H2O 1.98g MnCl2.4H2O Adjust to pH 5.8 with 0.2 M acetic acid Add dH2O to 200mL Filter sterilize Store refrigerated at 4°C	2.5g Yeast Extract 10g Tryptone 5g MgSO4.7H2O Adjust pH to 7.5 with KOH For Plates: add 7.5g of Agar

References

iGEM Toulouse 2015

INSA Toulouse
135, Avenue de Rangueil
31400 TOULOUSE
France

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