Team:Aalto-Helsinki/Experiments

• Project
• Modeling
  • Models of propane pathway
    • Kinetics of propane pathway
    • Model of CAR activation
  • Synergy model
  • Micelle model
  • About cellulose pathway
• Laboratory
  • Lab Book
  • Protocols
  • Safety
  • Practice project
• Results
  • Continuous production
  • Submitted parts
  • Achievements
  • Future
• Practices
  • Combining modeling and experimentation in iGEM
  • Outreach
• Community
  • Collaboration
  • Collaboration Platform
  • Collab seeker
  • Interlab study
    • InterLabBook
• People
  • Team
  • Attributions
• Journal
• 
• 

Protocols

Describe the experiments, research and protocols you used in your iGEM project.

Preparations

• Chemically Competent Cells
• Chloramphenicol plates
• Glycerol Stock of Bacteria
• PCR
• SDS-PAGE
• Transformation
• Tetracyclin plates
• Terrific Broth Media
• TOPO Cloning

Assembly

• ELIC
• Ligation
• NEBuilder
• OE-PCR
• Restriction

Measurements

• Blue Chromoprotein
• Vial-Scale Propane Gas Chomatography

Inspiration

• 2014 Colombia
• 2014 Imperial
• 2014 Caltech