Team:Birkbeck/InterLab Study/ungrouped standardized OD600

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Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




ANOVA of OD600 between strains of E. coli ungrouped1.htm

ANOVA Table of Ungrouped E. coli DH5α Standardised OD600.
DilutionOD600
  Sum of Squares df Mean Square F Sig.
Between Groups .005 17 .000 1.577 .123
Within Groups .007 36 .000    
Total .012 53      

Multiple Comparisons of Ungrouped E. coli DH5α Standardised OD600.
DilutionOD600
Tukey HSD
(I) GroupingTtest (J) GroupingTtest Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha (first triplicate) E. coli DH5 alpha (second triplicate) -.028667 .011482 .547 -.07173 .01440
E. coli DH5 alpha (third triplicate) -.008667 .011482 1.000 -.05173 .03440
E. coli DH5 alpha P1 (first triplicate) -.026667 .011482 .663 -.06973 .01640
E. coli DH5 alpha P1 (second triplicate) -.015000 .011482 .996 -.05806 .02806
E. coli DH5 alpha P1 (third triplicate) -.006333 .011482 1.000 -.04940 .03673
E. coli DH5 alpha P2 (first triplicate) -.008000 .011482 1.000 -.05106 .03506
E. coli DH5 alpha P2 (second triplicate) -.003667 .011482 1.000 -.04673 .03940
E. coli DH5 alpha P2 (third triplicate) -.014667 .011482 .997 -.05773 .02840
E. coli DH5 alpha P3 (first triplicate) -.006667 .011482 1.000 -.04973 .03640
E. coli DH5 alpha P3 (second triplicate) -.036000 .011482 .195 -.07906 .00706
E. coli DH5 alpha P3 (third triplicate) -.017000 .011482 .987 -.06006 .02606
E. coli DH5 alpha Positive control (first triplicate) -.012000 .011482 1.000 -.05506 .03106
E. coli DH5 alpha Positive control (second triplicate) -.006667 .011482 1.000 -.04973 .03640
E. coli DH5 alpha Positive control (third triplicate) -.011667 .011482 1.000 -.05473 .03140
E. coli DH5 alpha negative control (first triplicate) -.024333 .011482 .789 -.06740 .01873
E. coli DH5 alpha negative control (second triplicate) -.015333 .011482 .995 -.05840 .02773
E. coli DH5 alpha negative control (third triplicate) -.030333 .011482 .452 -.07340 .01273
E. coli DH5 alpha (second triplicate) E. coli DH5 alpha (first triplicate) .028667 .011482 .547 -.01440 .07173
E. coli DH5 alpha (third triplicate) .020000 .011482 .944 -.02306 .06306
E. coli DH5 alpha P1 (first triplicate) .002000 .011482 1.000 -.04106 .04506
E. coli DH5 alpha P1 (second triplicate) .013667 .011482 .999 -.02940 .05673
E. coli DH5 alpha P1 (third triplicate) .022333 .011482 .875 -.02073 .06540
E. coli DH5 alpha P2 (first triplicate) .020667 .011482 .928 -.02240 .06373
E. coli DH5 alpha P2 (second triplicate) .025000 .011482 .755 -.01806 .06806
E. coli DH5 alpha P2 (third triplicate) .014000 .011482 .998 -.02906 .05706
E. coli DH5 alpha P3 (first triplicate) .022000 .011482 .887 -.02106 .06506
E. coli DH5 alpha P3 (second triplicate) -.007333 .011482 1.000 -.05040 .03573
E. coli DH5 alpha P3 (third triplicate) .011667 .011482 1.000 -.03140 .05473
E. coli DH5 alpha Positive control (first triplicate) .016667 .011482 .989 -.02640 .05973
E. coli DH5 alpha Positive control (second triplicate) .022000 .011482 .887 -.02106 .06506
E. coli DH5 alpha Positive control (third triplicate) .017000 .011482 .987 -.02606 .06006
E. coli DH5 alpha negative control (first triplicate) .004333 .011482 1.000 -.03873 .04740
E. coli DH5 alpha negative control (second triplicate) .013333 .011482 .999 -.02973 .05640
E. coli DH5 alpha negative control (third triplicate) -.001667 .011482 1.000 -.04473 .04140
E. coli DH5 alpha (third triplicate) E. coli DH5 alpha (first triplicate) .008667 .011482 1.000 -.03440 .05173
E. coli DH5 alpha (second triplicate) -.020000 .011482 .944 -.06306 .02306
E. coli DH5 alpha P1 (first triplicate) -.018000 .011482 .977 -.06106 .02506
E. coli DH5 alpha P1 (second triplicate) -.006333 .011482 1.000 -.04940 .03673
E. coli DH5 alpha P1 (third triplicate) .002333 .011482 1.000 -.04073 .04540
E. coli DH5 alpha P2 (first triplicate) .000667 .011482 1.000 -.04240 .04373
E. coli DH5 alpha P2 (second triplicate) .005000 .011482 1.000 -.03806 .04806
E. coli DH5 alpha P2 (third triplicate) -.006000 .011482 1.000 -.04906 .03706
E. coli DH5 alpha P3 (first triplicate) .002000 .011482 1.000 -.04106 .04506
E. coli DH5 alpha P3 (second triplicate) -.027333 .011482 .625 -.07040 .01573
E. coli DH5 alpha P3 (third triplicate) -.008333 .011482 1.000 -.05140 .03473
E. coli DH5 alpha Positive control (first triplicate) -.003333 .011482 1.000 -.04640 .03973
E. coli DH5 alpha Positive control (second triplicate) .002000 .011482 1.000 -.04106 .04506
E. coli DH5 alpha Positive control (third triplicate) -.003000 .011482 1.000 -.04606 .04006
E. coli DH5 alpha negative control (first triplicate) -.015667 .011482 .994 -.05873 .02740
E. coli DH5 alpha negative control (second triplicate) -.006667 .011482 1.000 -.04973 .03640
E. coli DH5 alpha negative control (third triplicate) -.021667 .011482 .899 -.06473 .02140
E. coli DH5 alpha P1 (first triplicate) E. coli DH5 alpha (first triplicate) .026667 .011482 .663 -.01640 .06973
E. coli DH5 alpha (second triplicate) -.002000 .011482 1.000 -.04506 .04106
E. coli DH5 alpha (third triplicate) .018000 .011482 .977 -.02506 .06106
E. coli DH5 alpha P1 (second triplicate) .011667 .011482 1.000 -.03140 .05473
E. coli DH5 alpha P1 (third triplicate) .020333 .011482 .937 -.02273 .06340
E. coli DH5 alpha P2 (first triplicate) .018667 .011482 .969 -.02440 .06173
E. coli DH5 alpha P2 (second triplicate) .023000 .011482 .849 -.02006 .06606
E. coli DH5 alpha P2 (third triplicate) .012000 .011482 1.000 -.03106 .05506
E. coli DH5 alpha P3 (first triplicate) .020000 .011482 .944 -.02306 .06306
E. coli DH5 alpha P3 (second triplicate) -.009333 .011482 1.000 -.05240 .03373
E. coli DH5 alpha P3 (third triplicate) .009667 .011482 1.000 -.03340 .05273
E. coli DH5 alpha Positive control (first triplicate) .014667 .011482 .997 -.02840 .05773
E. coli DH5 alpha Positive control (second triplicate) .020000 .011482 .944 -.02306 .06306
E. coli DH5 alpha Positive control (third triplicate) .015000 .011482 .996 -.02806 .05806
E. coli DH5 alpha negative control (first triplicate) .002333 .011482 1.000 -.04073 .04540
E. coli DH5 alpha negative control (second triplicate) .011333 .011482 1.000 -.03173 .05440
E. coli DH5 alpha negative control (third triplicate) -.003667 .011482 1.000 -.04673 .03940
E. coli DH5 alpha P1 (second triplicate) E. coli DH5 alpha (first triplicate) .015000 .011482 .996 -.02806 .05806
E. coli DH5 alpha (second triplicate) -.013667 .011482 .999 -.05673 .02940
E. coli DH5 alpha (third triplicate) .006333 .011482 1.000 -.03673 .04940
E. coli DH5 alpha P1 (first triplicate) -.011667 .011482 1.000 -.05473 .03140
E. coli DH5 alpha P1 (third triplicate) .008667 .011482 1.000 -.03440 .05173
E. coli DH5 alpha P2 (first triplicate) .007000 .011482 1.000 -.03606 .05006
E. coli DH5 alpha P2 (second triplicate) .011333 .011482 1.000 -.03173 .05440
E. coli DH5 alpha P2 (third triplicate) .000333 .011482 1.000 -.04273 .04340
E. coli DH5 alpha P3 (first triplicate) .008333 .011482 1.000 -.03473 .05140
E. coli DH5 alpha P3 (second triplicate) -.021000 .011482 .919 -.06406 .02206
E. coli DH5 alpha P3 (third triplicate) -.002000 .011482 1.000 -.04506 .04106
E. coli DH5 alpha Positive control (first triplicate) .003000 .011482 1.000 -.04006 .04606
E. coli DH5 alpha Positive control (second triplicate) .008333 .011482 1.000 -.03473 .05140
E. coli DH5 alpha Positive control (third triplicate) .003333 .011482 1.000 -.03973 .04640
E. coli DH5 alpha negative control (first triplicate) -.009333 .011482 1.000 -.05240 .03373
E. coli DH5 alpha negative control (second triplicate) -.000333 .011482 1.000 -.04340 .04273
E. coli DH5 alpha negative control (third triplicate) -.015333 .011482 .995 -.05840 .02773
E. coli DH5 alpha P1 (third triplicate) E. coli DH5 alpha (first triplicate) .006333 .011482 1.000 -.03673 .04940
E. coli DH5 alpha (second triplicate) -.022333 .011482 .875 -.06540 .02073
E. coli DH5 alpha (third triplicate) -.002333 .011482 1.000 -.04540 .04073
E. coli DH5 alpha P1 (first triplicate) -.020333 .011482 .937 -.06340 .02273
E. coli DH5 alpha P1 (second triplicate) -.008667 .011482 1.000 -.05173 .03440
E. coli DH5 alpha P2 (first triplicate) -.001667 .011482 1.000 -.04473 .04140
E. coli DH5 alpha P2 (second triplicate) .002667 .011482 1.000 -.04040 .04573
E. coli DH5 alpha P2 (third triplicate) -.008333 .011482 1.000 -.05140 .03473
E. coli DH5 alpha P3 (first triplicate) -.000333 .011482 1.000 -.04340 .04273
E. coli DH5 alpha P3 (second triplicate) -.029667 .011482 .489 -.07273 .01340
E. coli DH5 alpha P3 (third triplicate) -.010667 .011482 1.000 -.05373 .03240
E. coli DH5 alpha Positive control (first triplicate) -.005667 .011482 1.000 -.04873 .03740
E. coli DH5 alpha Positive control (second triplicate) -.000333 .011482 1.000 -.04340 .04273
E. coli DH5 alpha Positive control (third triplicate) -.005333 .011482 1.000 -.04840 .03773
E. coli DH5 alpha negative control (first triplicate) -.018000 .011482 .977 -.06106 .02506
E. coli DH5 alpha negative control (second triplicate) -.009000 .011482 1.000 -.05206 .03406
E. coli DH5 alpha negative control (third triplicate) -.024000 .011482 .805 -.06706 .01906
E. coli DH5 alpha P2 (first triplicate) E. coli DH5 alpha (first triplicate) .008000 .011482 1.000 -.03506 .05106
E. coli DH5 alpha (second triplicate) -.020667 .011482 .928 -.06373 .02240
E. coli DH5 alpha (third triplicate) -.000667 .011482 1.000 -.04373 .04240
E. coli DH5 alpha P1 (first triplicate) -.018667 .011482 .969 -.06173 .02440
E. coli DH5 alpha P1 (second triplicate) -.007000 .011482 1.000 -.05006 .03606
E. coli DH5 alpha P1 (third triplicate) .001667 .011482 1.000 -.04140 .04473
E. coli DH5 alpha P2 (second triplicate) .004333 .011482 1.000 -.03873 .04740
E. coli DH5 alpha P2 (third triplicate) -.006667 .011482 1.000 -.04973 .03640
E. coli DH5 alpha P3 (first triplicate) .001333 .011482 1.000 -.04173 .04440
E. coli DH5 alpha P3 (second triplicate) -.028000 .011482 .586 -.07106 .01506
E. coli DH5 alpha P3 (third triplicate) -.009000 .011482 1.000 -.05206 .03406
E. coli DH5 alpha Positive control (first triplicate) -.004000 .011482 1.000 -.04706 .03906
E. coli DH5 alpha Positive control (second triplicate) .001333 .011482 1.000 -.04173 .04440
E. coli DH5 alpha Positive control (third triplicate) -.003667 .011482 1.000 -.04673 .03940
E. coli DH5 alpha negative control (first triplicate) -.016333 .011482 .991 -.05940 .02673
E. coli DH5 alpha negative control (second triplicate) -.007333 .011482 1.000 -.05040 .03573
E. coli DH5 alpha negative control (third triplicate) -.022333 .011482 .875 -.06540 .02073
E. coli DH5 alpha P2 (second triplicate) E. coli DH5 alpha (first triplicate) .003667 .011482 1.000 -.03940 .04673
E. coli DH5 alpha (second triplicate) -.025000 .011482 .755 -.06806 .01806
E. coli DH5 alpha (third triplicate) -.005000 .011482 1.000 -.04806 .03806
E. coli DH5 alpha P1 (first triplicate) -.023000 .011482 .849 -.06606 .02006
E. coli DH5 alpha P1 (second triplicate) -.011333 .011482 1.000 -.05440 .03173
E. coli DH5 alpha P1 (third triplicate) -.002667 .011482 1.000 -.04573 .04040
E. coli DH5 alpha P2 (first triplicate) -.004333 .011482 1.000 -.04740 .03873
E. coli DH5 alpha P2 (third triplicate) -.011000 .011482 1.000 -.05406 .03206
E. coli DH5 alpha P3 (first triplicate) -.003000 .011482 1.000 -.04606 .04006
E. coli DH5 alpha P3 (second triplicate) -.032333 .011482 .347 -.07540 .01073
E. coli DH5 alpha P3 (third triplicate) -.013333 .011482 .999 -.05640 .02973
E. coli DH5 alpha Positive control (first triplicate) -.008333 .011482 1.000 -.05140 .03473
E. coli DH5 alpha Positive control (second triplicate) -.003000 .011482 1.000 -.04606 .04006
E. coli DH5 alpha Positive control (third triplicate) -.008000 .011482 1.000 -.05106 .03506
E. coli DH5 alpha negative control (first triplicate) -.020667 .011482 .928 -.06373 .02240
E. coli DH5 alpha negative control (second triplicate) -.011667 .011482 1.000 -.05473 .03140
E. coli DH5 alpha negative control (third triplicate) -.026667 .011482 .663 -.06973 .01640
E. coli DH5 alpha P2 (third triplicate) E. coli DH5 alpha (first triplicate) .014667 .011482 .997 -.02840 .05773
E. coli DH5 alpha (second triplicate) -.014000 .011482 .998 -.05706 .02906
E. coli DH5 alpha (third triplicate) .006000 .011482 1.000 -.03706 .04906
E. coli DH5 alpha P1 (first triplicate) -.012000 .011482 1.000 -.05506 .03106
E. coli DH5 alpha P1 (second triplicate) -.000333 .011482 1.000 -.04340 .04273
E. coli DH5 alpha P1 (third triplicate) .008333 .011482 1.000 -.03473 .05140
E. coli DH5 alpha P2 (first triplicate) .006667 .011482 1.000 -.03640 .04973
E. coli DH5 alpha P2 (second triplicate) .011000 .011482 1.000 -.03206 .05406
E. coli DH5 alpha P3 (first triplicate) .008000 .011482 1.000 -.03506 .05106
E. coli DH5 alpha P3 (second triplicate) -.021333 .011482 .909 -.06440 .02173
E. coli DH5 alpha P3 (third triplicate) -.002333 .011482 1.000 -.04540 .04073
E. coli DH5 alpha Positive control (first triplicate) .002667 .011482 1.000 -.04040 .04573
E. coli DH5 alpha Positive control (second triplicate) .008000 .011482 1.000 -.03506 .05106
E. coli DH5 alpha Positive control (third triplicate) .003000 .011482 1.000 -.04006 .04606
E. coli DH5 alpha negative control (first triplicate) -.009667 .011482 1.000 -.05273 .03340
E. coli DH5 alpha negative control (second triplicate) -.000667 .011482 1.000 -.04373 .04240
E. coli DH5 alpha negative control (third triplicate) -.015667 .011482 .994 -.05873 .02740
E. coli DH5 alpha P3 (first triplicate) E. coli DH5 alpha (first triplicate) .006667 .011482 1.000 -.03640 .04973
E. coli DH5 alpha (second triplicate) -.022000 .011482 .887 -.06506 .02106
E. coli DH5 alpha (third triplicate) -.002000 .011482 1.000 -.04506 .04106
E. coli DH5 alpha P1 (first triplicate) -.020000 .011482 .944 -.06306 .02306
E. coli DH5 alpha P1 (second triplicate) -.008333 .011482 1.000 -.05140 .03473
E. coli DH5 alpha P1 (third triplicate) .000333 .011482 1.000 -.04273 .04340
E. coli DH5 alpha P2 (first triplicate) -.001333 .011482 1.000 -.04440 .04173
E. coli DH5 alpha P2 (second triplicate) .003000 .011482 1.000 -.04006 .04606
E. coli DH5 alpha P2 (third triplicate) -.008000 .011482 1.000 -.05106 .03506
E. coli DH5 alpha P3 (second triplicate) -.029333 .011482 .508 -.07240 .01373
E. coli DH5 alpha P3 (third triplicate) -.010333 .011482 1.000 -.05340 .03273
E. coli DH5 alpha Positive control (first triplicate) -.005333 .011482 1.000 -.04840 .03773
E. coli DH5 alpha Positive control (second triplicate) .000000 .011482 1.000 -.04306 .04306
E. coli DH5 alpha Positive control (third triplicate) -.005000 .011482 1.000 -.04806 .03806
E. coli DH5 alpha negative control (first triplicate) -.017667 .011482 .981 -.06073 .02540
E. coli DH5 alpha negative control (second triplicate) -.008667 .011482 1.000 -.05173 .03440
E. coli DH5 alpha negative control (third triplicate) -.023667 .011482 .820 -.06673 .01940
E. coli DH5 alpha P3 (second triplicate) E. coli DH5 alpha (first triplicate) .036000 .011482 .195 -.00706 .07906
E. coli DH5 alpha (second triplicate) .007333 .011482 1.000 -.03573 .05040
E. coli DH5 alpha (third triplicate) .027333 .011482 .625 -.01573 .07040
E. coli DH5 alpha P1 (first triplicate) .009333 .011482 1.000 -.03373 .05240
E. coli DH5 alpha P1 (second triplicate) .021000 .011482 .919 -.02206 .06406
E. coli DH5 alpha P1 (third triplicate) .029667 .011482 .489 -.01340 .07273
E. coli DH5 alpha P2 (first triplicate) .028000 .011482 .586 -.01506 .07106
E. coli DH5 alpha P2 (second triplicate) .032333 .011482 .347 -.01073 .07540
E. coli DH5 alpha P2 (third triplicate) .021333 .011482 .909 -.02173 .06440
E. coli DH5 alpha P3 (first triplicate) .029333 .011482 .508 -.01373 .07240
E. coli DH5 alpha P3 (third triplicate) .019000 .011482 .964 -.02406 .06206
E. coli DH5 alpha Positive control (first triplicate) .024000 .011482 .805 -.01906 .06706
E. coli DH5 alpha Positive control (second triplicate) .029333 .011482 .508 -.01373 .07240
E. coli DH5 alpha Positive control (third triplicate) .024333 .011482 .789 -.01873 .06740
E. coli DH5 alpha negative control (first triplicate) .011667 .011482 1.000 -.03140 .05473
E. coli DH5 alpha negative control (second triplicate) .020667 .011482 .928 -.02240 .06373
E. coli DH5 alpha negative control (third triplicate) .005667 .011482 1.000 -.03740 .04873
E. coli DH5 alpha P3 (third triplicate) E. coli DH5 alpha (first triplicate) .017000 .011482 .987 -.02606 .06006
E. coli DH5 alpha (second triplicate) -.011667 .011482 1.000 -.05473 .03140
E. coli DH5 alpha (third triplicate) .008333 .011482 1.000 -.03473 .05140
E. coli DH5 alpha P1 (first triplicate) -.009667 .011482 1.000 -.05273 .03340
E. coli DH5 alpha P1 (second triplicate) .002000 .011482 1.000 -.04106 .04506
E. coli DH5 alpha P1 (third triplicate) .010667 .011482 1.000 -.03240 .05373
E. coli DH5 alpha P2 (first triplicate) .009000 .011482 1.000 -.03406 .05206
E. coli DH5 alpha P2 (second triplicate) .013333 .011482 .999 -.02973 .05640
E. coli DH5 alpha P2 (third triplicate) .002333 .011482 1.000 -.04073 .04540
E. coli DH5 alpha P3 (first triplicate) .010333 .011482 1.000 -.03273 .05340
E. coli DH5 alpha P3 (second triplicate) -.019000 .011482 .964 -.06206 .02406
E. coli DH5 alpha Positive control (first triplicate) .005000 .011482 1.000 -.03806 .04806
E. coli DH5 alpha Positive control (second triplicate) .010333 .011482 1.000 -.03273 .05340
E. coli DH5 alpha Positive control (third triplicate) .005333 .011482 1.000 -.03773 .04840
E. coli DH5 alpha negative control (first triplicate) -.007333 .011482 1.000 -.05040 .03573
E. coli DH5 alpha negative control (second triplicate) .001667 .011482 1.000 -.04140 .04473
E. coli DH5 alpha negative control (third triplicate) -.013333 .011482 .999 -.05640 .02973
E. coli DH5 alpha Positive control (first triplicate) E. coli DH5 alpha (first triplicate) .012000 .011482 1.000 -.03106 .05506
E. coli DH5 alpha (second triplicate) -.016667 .011482 .989 -.05973 .02640
E. coli DH5 alpha (third triplicate) .003333 .011482 1.000 -.03973 .04640
E. coli DH5 alpha P1 (first triplicate) -.014667 .011482 .997 -.05773 .02840
E. coli DH5 alpha P1 (second triplicate) -.003000 .011482 1.000 -.04606 .04006
E. coli DH5 alpha P1 (third triplicate) .005667 .011482 1.000 -.03740 .04873
E. coli DH5 alpha P2 (first triplicate) .004000 .011482 1.000 -.03906 .04706
E. coli DH5 alpha P2 (second triplicate) .008333 .011482 1.000 -.03473 .05140
E. coli DH5 alpha P2 (third triplicate) -.002667 .011482 1.000 -.04573 .04040
E. coli DH5 alpha P3 (first triplicate) .005333 .011482 1.000 -.03773 .04840
E. coli DH5 alpha P3 (second triplicate) -.024000 .011482 .805 -.06706 .01906
E. coli DH5 alpha P3 (third triplicate) -.005000 .011482 1.000 -.04806 .03806
E. coli DH5 alpha Positive control (second triplicate) .005333 .011482 1.000 -.03773 .04840
E. coli DH5 alpha Positive control (third triplicate) .000333 .011482 1.000 -.04273 .04340
E. coli DH5 alpha negative control (first triplicate) -.012333 .011482 1.000 -.05540 .03073
E. coli DH5 alpha negative control (second triplicate) -.003333 .011482 1.000 -.04640 .03973
E. coli DH5 alpha negative control (third triplicate) -.018333 .011482 .973 -.06140 .02473
E. coli DH5 alpha Positive control (second triplicate) E. coli DH5 alpha (first triplicate) .006667 .011482 1.000 -.03640 .04973
E. coli DH5 alpha (second triplicate) -.022000 .011482 .887 -.06506 .02106
E. coli DH5 alpha (third triplicate) -.002000 .011482 1.000 -.04506 .04106
E. coli DH5 alpha P1 (first triplicate) -.020000 .011482 .944 -.06306 .02306
E. coli DH5 alpha P1 (second triplicate) -.008333 .011482 1.000 -.05140 .03473
E. coli DH5 alpha P1 (third triplicate) .000333 .011482 1.000 -.04273 .04340
E. coli DH5 alpha P2 (first triplicate) -.001333 .011482 1.000 -.04440 .04173
E. coli DH5 alpha P2 (second triplicate) .003000 .011482 1.000 -.04006 .04606
E. coli DH5 alpha P2 (third triplicate) -.008000 .011482 1.000 -.05106 .03506
E. coli DH5 alpha P3 (first triplicate) .000000 .011482 1.000 -.04306 .04306
E. coli DH5 alpha P3 (second triplicate) -.029333 .011482 .508 -.07240 .01373
E. coli DH5 alpha P3 (third triplicate) -.010333 .011482 1.000 -.05340 .03273
E. coli DH5 alpha Positive control (first triplicate) -.005333 .011482 1.000 -.04840 .03773
E. coli DH5 alpha Positive control (third triplicate) -.005000 .011482 1.000 -.04806 .03806
E. coli DH5 alpha negative control (first triplicate) -.017667 .011482 .981 -.06073 .02540
E. coli DH5 alpha negative control (second triplicate) -.008667 .011482 1.000 -.05173 .03440
E. coli DH5 alpha negative control (third triplicate) -.023667 .011482 .820 -.06673 .01940
E. coli DH5 alpha Positive control (third triplicate) E. coli DH5 alpha (first triplicate) .011667 .011482 1.000 -.03140 .05473
E. coli DH5 alpha (second triplicate) -.017000 .011482 .987 -.06006 .02606
E. coli DH5 alpha (third triplicate) .003000 .011482 1.000 -.04006 .04606
E. coli DH5 alpha P1 (first triplicate) -.015000 .011482 .996 -.05806 .02806
E. coli DH5 alpha P1 (second triplicate) -.003333 .011482 1.000 -.04640 .03973
E. coli DH5 alpha P1 (third triplicate) .005333 .011482 1.000 -.03773 .04840
E. coli DH5 alpha P2 (first triplicate) .003667 .011482 1.000 -.03940 .04673
E. coli DH5 alpha P2 (second triplicate) .008000 .011482 1.000 -.03506 .05106
E. coli DH5 alpha P2 (third triplicate) -.003000 .011482 1.000 -.04606 .04006
E. coli DH5 alpha P3 (first triplicate) .005000 .011482 1.000 -.03806 .04806
E. coli DH5 alpha P3 (second triplicate) -.024333 .011482 .789 -.06740 .01873
E. coli DH5 alpha P3 (third triplicate) -.005333 .011482 1.000 -.04840 .03773
E. coli DH5 alpha Positive control (first triplicate) -.000333 .011482 1.000 -.04340 .04273
E. coli DH5 alpha Positive control (second triplicate) .005000 .011482 1.000 -.03806 .04806
E. coli DH5 alpha negative control (first triplicate) -.012667 .011482 1.000 -.05573 .03040
E. coli DH5 alpha negative control (second triplicate) -.003667 .011482 1.000 -.04673 .03940
E. coli DH5 alpha negative control (third triplicate) -.018667 .011482 .969 -.06173 .02440
E. coli DH5 alpha negative control (first triplicate) E. coli DH5 alpha (first triplicate) .024333 .011482 .789 -.01873 .06740
E. coli DH5 alpha (second triplicate) -.004333 .011482 1.000 -.04740 .03873
E. coli DH5 alpha (third triplicate) .015667 .011482 .994 -.02740 .05873
E. coli DH5 alpha P1 (first triplicate) -.002333 .011482 1.000 -.04540 .04073
E. coli DH5 alpha P1 (second triplicate) .009333 .011482 1.000 -.03373 .05240
E. coli DH5 alpha P1 (third triplicate) .018000 .011482 .977 -.02506 .06106
E. coli DH5 alpha P2 (first triplicate) .016333 .011482 .991 -.02673 .05940
E. coli DH5 alpha P2 (second triplicate) .020667 .011482 .928 -.02240 .06373
E. coli DH5 alpha P2 (third triplicate) .009667 .011482 1.000 -.03340 .05273
E. coli DH5 alpha P3 (first triplicate) .017667 .011482 .981 -.02540 .06073
E. coli DH5 alpha P3 (second triplicate) -.011667 .011482 1.000 -.05473 .03140
E. coli DH5 alpha P3 (third triplicate) .007333 .011482 1.000 -.03573 .05040
E. coli DH5 alpha Positive control (first triplicate) .012333 .011482 1.000 -.03073 .05540
E. coli DH5 alpha Positive control (second triplicate) .017667 .011482 .981 -.02540 .06073
E. coli DH5 alpha Positive control (third triplicate) .012667 .011482 1.000 -.03040 .05573
E. coli DH5 alpha negative control (second triplicate) .009000 .011482 1.000 -.03406 .05206
E. coli DH5 alpha negative control (third triplicate) -.006000 .011482 1.000 -.04906 .03706
E. coli DH5 alpha negative control (second triplicate) E. coli DH5 alpha (first triplicate) .015333 .011482 .995 -.02773 .05840
E. coli DH5 alpha (second triplicate) -.013333 .011482 .999 -.05640 .02973
E. coli DH5 alpha (third triplicate) .006667 .011482 1.000 -.03640 .04973
E. coli DH5 alpha P1 (first triplicate) -.011333 .011482 1.000 -.05440 .03173
E. coli DH5 alpha P1 (second triplicate) .000333 .011482 1.000 -.04273 .04340
E. coli DH5 alpha P1 (third triplicate) .009000 .011482 1.000 -.03406 .05206
E. coli DH5 alpha P2 (first triplicate) .007333 .011482 1.000 -.03573 .05040
E. coli DH5 alpha P2 (second triplicate) .011667 .011482 1.000 -.03140 .05473
E. coli DH5 alpha P2 (third triplicate) .000667 .011482 1.000 -.04240 .04373
E. coli DH5 alpha P3 (first triplicate) .008667 .011482 1.000 -.03440 .05173
E. coli DH5 alpha P3 (second triplicate) -.020667 .011482 .928 -.06373 .02240
E. coli DH5 alpha P3 (third triplicate) -.001667 .011482 1.000 -.04473 .04140
E. coli DH5 alpha Positive control (first triplicate) .003333 .011482 1.000 -.03973 .04640
E. coli DH5 alpha Positive control (second triplicate) .008667 .011482 1.000 -.03440 .05173
E. coli DH5 alpha Positive control (third triplicate) .003667 .011482 1.000 -.03940 .04673
E. coli DH5 alpha negative control (first triplicate) -.009000 .011482 1.000 -.05206 .03406
E. coli DH5 alpha negative control (third triplicate) -.015000 .011482 .996 -.05806 .02806
E. coli DH5 alpha negative control (third triplicate) E. coli DH5 alpha (first triplicate) .030333 .011482 .452 -.01273 .07340
E. coli DH5 alpha (second triplicate) .001667 .011482 1.000 -.04140 .04473
E. coli DH5 alpha (third triplicate) .021667 .011482 .899 -.02140 .06473
E. coli DH5 alpha P1 (first triplicate) .003667 .011482 1.000 -.03940 .04673
E. coli DH5 alpha P1 (second triplicate) .015333 .011482 .995 -.02773 .05840
E. coli DH5 alpha P1 (third triplicate) .024000 .011482 .805 -.01906 .06706
E. coli DH5 alpha P2 (first triplicate) .022333 .011482 .875 -.02073 .06540
E. coli DH5 alpha P2 (second triplicate) .026667 .011482 .663 -.01640 .06973
E. coli DH5 alpha P2 (third triplicate) .015667 .011482 .994 -.02740 .05873
E. coli DH5 alpha P3 (first triplicate) .023667 .011482 .820 -.01940 .06673
E. coli DH5 alpha P3 (second triplicate) -.005667 .011482 1.000 -.04873 .03740
E. coli DH5 alpha P3 (third triplicate) .013333 .011482 .999 -.02973 .05640
E. coli DH5 alpha Positive control (first triplicate) .018333 .011482 .973 -.02473 .06140
E. coli DH5 alpha Positive control (second triplicate) .023667 .011482 .820 -.01940 .06673
E. coli DH5 alpha Positive control (third triplicate) .018667 .011482 .969 -.02440 .06173
E. coli DH5 alpha negative control (first triplicate) .006000 .011482 1.000 -.03706 .04906
E. coli DH5 alpha negative control (second triplicate) .015000 .011482 .996 -.02806 .05806