Team:DTU-Denmark/Journal
Summary
Methods
Gel electrophoresis
Genomic DNA extraction (B. brevis)
Gibson Assembly
Glycerol stock –preservation
Ligation of DNA
PCR -
- PCR
- Colony PCR
- Touch down
- …
Plasmid Purification Miniprep
Restriction enzyme digest (NEB)
Transformation and Preparation of E.coli
-Preparation of Competent Cells
-Transformation of Chemically Competent E.coli
Transformation of Bacillus
Timeline
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Visit from Fisher Scientific!
2015-08-26
Today we had a visit by Susanne Basse from Fisher Scientific. She came by our office with a wagon full of items, Thanks!
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Contribution from Macrogen!
2015-08-20
We are pleased to announce that Macrogen is sponsoring our team with sequencing!
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Contributions from AKG!
2015-08-19
We are happy to announce that AKG Acoustics is sponsoring our team with headphones for presentations in Boston!
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Contributions from VWR - Bie & Berntsen A/S
2015-08-11
We are happy to announce that VWR - Bie & Berntsen A/S is sponsoring our team with lab consumeables!
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Contributions from Fisher Scientific
2015-08-11
We are happy to announce that Fisher Scientific is sponsoring our team with lab materials!
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Canoeing
2015-08-07
Today we took a day off from lab to go canoeing, followed by a barbeque at Chris' place.
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Contribution from SnapGene
2015-08-03
We are happy to announce that the SnapGene is contributing our team with lisences to their awesome product!
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Wiki Wizard
2015-07-30
We have uploaded the first beta version of the Wiki Wizard to GitHub
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First BioBrick cloned into B. subtilis
2015-07-30
An expression cassette with lambda beta recombinase was sucesfully transformed into Bacillus subtilis. First step towards establishing MAGE in Bacillus subtilis completed!
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BioBrick High School Project
2015-07-23
3 high school students; Simran, Noor, and Charlotte, joined us and Biotech Academy for 5 days to make biosensors that changes color, when water is poluted. Read more at ADD and http://biotechacademy.dk.
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First sequencing results
2015-07-22
We received our first sequencing results. One step closer to our first BioBrick!
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Brevibacillus parabrevis arrives
2015-07-16
Brevibacillus parabrevis produces a lot of different antibiotics including tyrocidine. We will transfer the tyrocidine operon to our chassis Bacillus subtilis.
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Contribution from the Lundbeck Foundation!
2015-07-15
We are happy to announce that the Lundbeck Foundation is contributing financially to our team!
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Contributions from In Vitro!
2015-05-06
We are happy to announce that In Vitro A/S is sponsoring our team with lab consumeables!
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Contributions from Frisnette
2015-04-30
We are happy to announce that Frisnette is sponsoring our team with lab consumeables!
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We get our strain
2015-04-28
We get a plate of our chassis strain Bacillus subtilis W168.
We can now begin the work in the laboratory. -
BioBrick Workshop
2015-04-24
Southern University of Denmark and University of Copenhagen joined us for a three-day BioBrick tutorial, where we spent three days in the laboratory cloning, learning about iGEM, wiki design, and getting to know each other through social activities.
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Contribution from the Otto Mønsted Fonden!
2015-03-08
We are happy to announce that the Otto Mønsted Fonden is contributing financially to our team!
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First official team meeting
2015-01-14
Today we had our first official team meeting. We talked about the project and got to know each other.
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Introductory meeting
2014-11-25
This day, last years team held an introductory session about iGEM for interested people.
