Team:Leicester/Measurement
Overview
As a team we decided that we would do some extra work during our time doing the iGEM project this such that we entered to take part in the Interlab Measurement study. The study requires us to look at the fluorescence of given gene circuits made up of parts found in the 2015 Distribution Kits.
The study requires producing 3 devices each consisting of a promoter sequence (depicted as different coloured arrows on Figure 1) and Green Fluorescence Protein (GFP) intermediate (I13504). Each of the devices are shown below:
- Device 1 - J23101 + I13504
- Device 2 - J23106 + I13504
- Device 3 - J23117 + I13504
As part of the Interlab study, we then did extra credit where we had get biological and technical replicates of each device. They have been defined as such:
- Biological Replicate -
- Technical Replicate -
Methodology
In order to get the
Results
Fluorescence results for the Interlab study were obtained using the BMG LABTECH FLUOstar Omega Microplate Reader. We placed all the replicates (technical and biological) on a black based 96 well plate. Results were obtained in the format of arbitrary fluorescence unit/OD 600
Microplate Reader Settings
- Emission - EMS20
- Excitation - 485-12nm
- No. of flashes per well- 20 /ul
All results from each device were then placed in the format of graphs as such: