Team:Aalto-Helsinki/InterLabBook
Worked With |
WorkFlow |
June 22nd - |
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Preparations |
Transform |
o/n culture |
MinPrep |
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Use iGEM's |
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Assembly |
3A assembly |
Stored |
Worked With |
WorkFlow |
June 29th - |
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Preparations |
Transform |
o/n culture |
MinPrep |
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Assembly |
Transform |
D2 |
Refresh |
No GFP |
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No colonies |
Re-assemble |
Transform |
D2 |
o/n culture |
MiniPrep |
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No colonies |
Worked With |
WorkFlow |
July 6th - |
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Checking D2 |
Transform D2 |
GFP |
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Assembly |
Re-restrict |
Stored |
Worked With |
WorkFlow |
July 27th - |
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Assembly |
Ligations |
Transform |
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Checking |
Check AHD1 |
AHD4 & AHD5 |
Too little |
Re-restrict |
Stored |
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Assembly |
Ligations |
Transform |
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AHD1 & AHD3 |
Re-restrict |
Stored |
Worked With |
WorkFlow |
August 3rd - |
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Assembly |
Ligations |
Transform |
D1 |
o/n culture |
MiniPrep |
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No GFP |
Re-restrict |
Ligate & |
No GFP |
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Checking |
Mistake in AHD4 |
Check AHD4 |
Restriction OK |
Worked With |
WorkFlow |
August 12th - |
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Measurement |
Transform |
o/n cultures |
Measure GFP |
Due to the limited time we had to use for our project, we had to abort the interlab measurement study. We were able to construct two devices and measure the GFP from these. We were unable to build the device with J23106 promoter.
Our results can be found on our Interlab Study main page.