Team:Shiyan SY China/Project.html
You have to believe in yourself. That’s the secret of success.
—— Charles Chaplin
With increased agriculture activities around the world, it becomes a common practice to use pesticides to manage pest problem. Runoff can carry field pesticide into aquatic environment while wind can carry them to other fields, potentially affecting other species. Over time, repeated application increases pest resistance and facilitate the pest resurgence. Further, especially in China, toxic pesticide residues on green vegetable and fruits become a major public health problem.
In order to provide a solution, we design an engineered bacterium, secreting the OMP enzyme to degrade the common toxic pesticide residues. Its secretion is under the temperature control and can only be activated at specified temperature. To avoid the secondary pollution, a UV-induced suicide gene is inserted into the bacteria: upon exposure to UV or sunshine, the suicide procedure is induced. This purpose of the design is to remove toxic pesticide safely without affecting the environment.
The increasing environmental pollution and changeable climates make the insect pests on crops more serious. According to the statistical data from Chinese Academy of Agricultural Sciences, in recent years, there have been over seventy kinds of insect pests on vegetables and fruits. If these insect pests are not controlled, they will lead to total failures of crops, such as vegetables, fruits and grains. Faced with raging insect pests, the farmers have to constantly spray pesticides, and even use the high-toxic pesticides which are forbidden by our country. Therefore, as a result of the use of pesticides, a majority of vegetables and fruits contain pesticides above the permitted limits, but they still flow into thousands of families through channels such as vegetable markets, supermarkets and roadside stalls.
It is shown according to the statistics from World Health Organization, there are at least 500 000 pesticide poisoning accidents and 115 000 persons who die of pesticide poisoning annually all over the world. Moreover, over 85% of cancer cases and over 80 kinds of diseases are relevant to pesticide residues. In many big cities in China, the exceeding standard rate of pesticide residues on vegetables and fruits reached up to 47%.
In our project to build biological modules, reference the study design of this project comes from the igem previous years, a large number of information and information from other research teams on the rest of society, the information in this project, coordinated and integrated to form and project implementation content.
1) a strong promoter sequence used in this project, quoted from igem of BB_J23106.
2) RNA thermometer sequences used in this project, quoted from igem of BB_K115017.
3) organophosphorus pesticides used in this project degrading enzyme gene sequence opdA, quoted from igem of BB_K215090, BB_K215091 and BB_K1010008.
4) The project used an inducible promoter repair enzyme RecA (SOS) sequence, quoted from igem of BB_J22106.
5) ccdB suicide gene sequences used in this project, quoted from igem of BB_K145151 and BB_K1010007.
6) ompA signal peptide sequence used in this project, quoted from GenBank sequences numbered AJ617284.1.
We connect we the World magazine and publish the Igem monograph . In the magazine we discuss the safety, ethic, advantage and disadvantage of the synthetic biology. We record the process of our team, including the safety of the experiment and the what we learned during the experiment in the CAS. World magazine is established by the Liaoning province Shiyan high school’s international department., facing the entire school and the high school in the province,; therefore, through the magazine, we are able to share and discuss the igem with other students.
This part includes two individual DNA domains. 1. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of opdA gene and expression of opdA protein out of the host cell via an ompA peptide. This DNA fragment domain could be obtained by double-digested with EcoR I + Spe I. 2. A UV induced promoter (RecA SOS) followed with a suicide gene ccdB, which could lead to the death of host cell if exposed in UV. This DNA fragment domain could be obtained by double-digested with Xho I + Spe I. And the whole part DNA fragment could be obtained by double-digested with Xho I + EcoR I.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1667005 | DNA | OpdA encoding gene with ompA signal peptide | 1776 |
We do a new way spread the knowledge of biology to the public, and we update the dynamic of our team work.
