Team:Aalto-Helsinki/Experiments

• Project
• Modeling
  • Models of propane pathway
    • Kinetics of propane pathway
    • Model of CAR activation
  • Synergy model
  • Micelle model
  • About cellulose pathway
• Laboratory
  • Lab Book
  • Protocols
  • Safety
  • Practice project
• Results
  • Continuous production
  • Submitted parts
  • Achievements
  • Future
• Practices
  • Combining modeling and experimentation in iGEM
  • Outreach
• Community
  • Collaboration
  • Collaboration Platform
  • Collab seeker
  • Interlab study
    • InterLabBook
• People
  • Team
  • Attributions
• Journal
• 
• 

Protocols

You can find all of the protocols used in our project here as .pdf files. We based many of our protocols on what we found from research articles, openwetware.org, and other webpages. Some were given to us by 2014 Aalto-Helsinki team or researchers in Aalto University and VTT, the Techinical Research Center of Finland.

Preparations

• Chemically Competent Cells

• Chloramphenicol plates

• CMC Plates with IPTG

• CMC Plates with Congo Red

• Colony PCR

• Glycerol Stock of Bacteria

• PCR with KAPA

• PCR with Q5

• SDS-PAGE

• Transformation

• Tetracyclin plates

• Terrific Broth Media

• TOPO Cloning

Assembly

• ELIC

• Ligation

• NEBuilder

• OE-PCR

• Restriction Digestion

Measurements

• Blue Chromoprotein

• Vial-Scale Propane Gas Chomatography