Team:Paris Saclay/Notebook/August/27

Wednesday 26th August

Lab Work

Electrophoresis

by Audrey

Verification of PCR products:

• BBa_K1707013
• BBa_K1707019
• BBa_K1707020
• BBa_K1707030
• BBa_K1707035
• BBa_K1707036
• BBa_K1707021
• BBa_K1707027
• Controls (-)

Agarose gel 1%, Migration 115V

We can conclude that:

• BBa_K1707035 and BBa_K1707036 aren't OK, we will have to start again

All of the next one (+DMSO) are OK:

• BBa_K1707013
• BBa_K1707019
• BBa_K1707020
• BBa_K1707030
• BBa_K1707021
• BBa_K1707027

Digestion

by Audrey

PCR products of: (+DMSO)

• BBa_K1707013
• BBa_K1707019
• BBa_K1707020
• BBa_K1707030
• BBa_K1707021
• BBa_K1707027
• BBa_K1707036

Mix in each tube:

• 1 μL DpnI
• 45μL PCR Product
• 5,5 μL FastDigest Buffer
• 3,5 μL H2O

Incubation 1h30, 37°C

Digestion

by Audrey

Biobricks:

• BBa_K1707022

Mix:

• • 1 μL XbaI
  • 1 μL PstI
  • 15 μL plasmid
  • 2 μL FastDigest Buffer
  • 1 μL H2O

• BBa_K1707023

Mix:

• • 1 μL SpeI
  • 1 μL PstI
  • 15 μL Plasmid
  • 2 μL FastDigest Buffer
  • 1 μL H2O

Incubation 1h30, 37°C

Member present:

• Instructors: Claire
• Students: Audrey

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