Team:Paris Saclay/Notebook/August/27


Tuesday 27th August

Lab Work

Electrophoresis

by Audrey

Verification of PCR products:

  • BBa_K1707013
  • BBa_K1707019
  • BBa_K1707020
  • BBa_K1707030
  • BBa_K1707035
  • BBa_K1707036
  • BBa_K1707021
  • BBa_K1707027
  • Controls (-)

Agarose gel 1%, Migration 115V

We can conclude that:

  • BBa_K1707035 and BBa_K1707036 aren't OK, we will have to start again

All of the next one (+DMSO) are OK:

  • BBa_K1707013
  • BBa_K1707019
  • BBa_K1707020
  • BBa_K1707030
  • BBa_K1707021
  • BBa_K1707027

Digestion

by Audrey

PCR products of: (+DMSO)

  • BBa_K1707013
  • BBa_K1707019
  • BBa_K1707020
  • BBa_K1707030
  • BBa_K1707021
  • BBa_K1707027
  • BBa_K1707036

Mix in each tube:

  • 1 μL DpnI
  • 45μL PCR Product
  • 5,5 μL FastDigest Buffer
  • 3,5 μL H2O

Incubation 1h30, 37°C

Digestion

by Audrey

  • BBa_K1707022

Mix:

    • 1 μL XbaI
    • 1 μL PstI
    • 15 μL plasmid
    • 2 μL FastDigest Buffer
    • 1 μL H2O


  • BBa_K1707023

Mix:

    • 1 μL SpeI
    • 1 μL PstI
    • 15 μL Plasmid
    • 2 μL FastDigest Buffer
    • 1 μL H2O

Incubation 1h30, 37°C

Member present:

  • Instructors: Claire
  • Students: Audrey

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