Team:TecCEM/2015
January
- Week 1 and 2: the team selected five Project proposals to further develop. The team was subdivided in teams to do research and presentations.
- Week 3: The team discarded two projects and analised the feasability and impact of three project proposals.
- 28th: The team finally selected a the DNAbot as the Project to be developed for the competition.
- 31st: The team presented the Project objectives to the Faculty College of the Biotechnology and Chemical Engineering Faculty of ITESM CEM.
February
- An aptameric sequence was selected and software was evaluated.
- The bioinformatic design of the DNAbot was performed.
- Human Practices events were planned for the entire period.
March
- Molecular dynamics and docking with ligand were performed for our design.
- Methodology selection for our synthetic biology approach for production. Linker biobrick design.
- Planning for the Synthetic Biology Course.
- Participation with Project abstract for IWA Conference to present poster.
- 26th and 27: First Human Practices participation: 5to Día del Emprendedor to get our Project out there and try to make contact with potential investors.
April
- Plasmid design with linker and nanobot sequences. Selection of Rolling circle amplification as potential method of production.
- Preparation of sequences for synthesis.
- 27th, 28th, and 29th: 4th International Water Association Mexico Young Water Professionals Conference 2015 poster presentation of bioinformatic analysis of Project.
May
- 1st, 2nd, and 3rd iGEM Latinoamerica Meet up at Monterrey: hosted by Tec-Monterrey team.
- Week 1 and 2: Synthetic Biology Course final arrangements.
- Development of the overall Project methodolgy and protocols.
- Final designs of plasmids containing the DNAbot sequences.
- Final designs for synthesis of sequences.
- 30th: Beggining of Synthetic Biology Couse.
June
- Start of Interlab Study protocols.
- Synthetic Biology Course in the ITESM facilities.
July
- 13th to 17th: Experimental work at CINVESTAV for fluorescence analysis.
- Protein modelling development for Igem TecCEM-HS team.
- Commencement of work with lysostaphin: resuspension on iGEM Plate.
August
- Protein analysis for lysostaphin and sensitivity antibiogram.
- Biobricks arrived!
- Oligos for DNAbot arrived!
- Cloning of biobricks.
September
- Assymetrical PCR for DNAbot scaffold 1 construction.
- DNAbot assembly.
- Biobricks documentation and characterisation.
- DNAbot imaging by TEM.
- Wikifreeze