Team:TecCEM/2015

Bannerteccem.jpg












2 0 1 5


January

  • Week 1 and 2: the team selected five Project proposals to further develop. The team was subdivided in teams to do research and presentations.
  • Week 3: The team discarded two projects and analised the feasability and impact of three project proposals.
  • 28th: The team finally selected a the DNAbot as the Project to be developed for the competition.
  • 31st: The team presented the Project objectives to the Faculty College of the Biotechnology and Chemical Engineering Faculty of ITESM CEM.


February

  • An aptameric sequence was selected and software was evaluated.
  • The bioinformatic design of the DNAbot was performed.
  • Human Practices events were planned for the entire period.


March

  • Molecular dynamics and docking with ligand were performed for our design.
  • Methodology selection for our synthetic biology approach for production. Linker biobrick design.
  • Planning for the Synthetic Biology Course.
  • Participation with Project abstract for IWA Conference to present poster.
  • 26th and 27: First Human Practices participation: 5to Día del Emprendedor to get our Project out there and try to make contact with potential investors.


April

  • Plasmid design with linker and nanobot sequences. Selection of Rolling circle amplification as potential method of production.
  • Preparation of sequences for synthesis.
  • 27th, 28th, and 29th: 4th International Water Association Mexico Young Water Professionals Conference 2015 poster presentation of bioinformatic analysis of Project.


May

  • 1st, 2nd, and 3rd iGEM Latinoamerica Meet up at Monterrey: hosted by Tec-Monterrey team.
  • Week 1 and 2: Synthetic Biology Course final arrangements.
  • Development of the overall Project methodolgy and protocols.
  • Final designs of plasmids containing the DNAbot sequences.
  • Final designs for synthesis of sequences.
  • 30th: Beggining of Synthetic Biology Couse.


June

  • Start of Interlab Study protocols.
  • Synthetic Biology Course in the ITESM facilities.


July

  • 13th to 17th: Experimental work at CINVESTAV for fluorescence analysis.
  • Protein modelling development for Igem TecCEM-HS team.
  • Commencement of work with lysostaphin: resuspension on iGEM Plate.


August

  • Protein analysis for lysostaphin and sensitivity antibiogram.
  • Biobricks arrived!
  • Oligos for DNAbot arrived!
  • Cloning of biobricks.


September

  • Assymetrical PCR for DNAbot scaffold 1 construction.
  • DNAbot assembly.
  • Biobricks documentation and characterisation.
  • DNAbot imaging by TEM.
  • Wikifreeze