Team:Brasil-USP/Notebook/protocols

Protocols

Notebook

Protocols

Table of contents

• Circuit Assembly Protocols
• Calcium chloride transformation with heat shock in Escherichia coli DH5α
• Plasmid extraction
• Digestion of plasmidial DNA
• Ligation reaction (Cohesive ends)
• Agarose Gel Electrophoresis
• Directed mutagenesis PCR (for restriction site elimination) using a plasmid template for restriction site elimination
• PCR amplification (applied to lcp)
• PCR amplification for difficult amplicons (applied to roxA)
• Gibson assembly
• Characterization Protocols
• Pre-inoculum
• Plate Reader
• Flow Cytometer

In order t