Team:TU Darmstadt/Notebook/sec1/K1602029

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K1602029 - T7-B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (T7-B0034-His-GFP-Si4)

Bba_K1602028 was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. Colonies were screened using a colony PCR with VF2 and VR.