Bba_K1602027 was digested with XbaI and PstI and ligated into B0034-pSB1A2 and transformed into E. coli Top 10. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Positive colonies were inoculated and the plasmids were extracted
Figure 1 Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around 1.1 kbp. DNA marker: Quick-Load Purple 2-Log DNA Ladder.
