Team:HUST-China/Notebook
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![](https://static.igem.org/mediawiki/2015/8/80/White.png)
![](https://static.igem.org/mediawiki/2015/d/da/Maodian.png)
Standalization
Week 1 6.29-7.5
Week 2 7.6-7.12
Week 3 7.13-7.19
Week 4 7.20-7.26
Week 5 7.27-8.2
Week 6 8.3-8.9
PCR: More details, protocol WangJie Liu and JunJie Xu
![](https://static.igem.org/mediawiki/2015/0/09/Standar-1-form.png)
![](https://static.igem.org/mediawiki/2015/f/f6/Standar-1-form-1-pic.png)
![](https://static.igem.org/mediawiki/2015/c/c7/Standar-1-form-2-pic.png)
![](https://static.igem.org/mediawiki/2015/8/8b/Standar-1-form-3-pic.png)
Restriction Enzyme Digestion
WangJie Liu and JunJie Xu
![](https://static.igem.org/mediawiki/2015/4/4c/Standar-2-form.png)
![](https://static.igem.org/mediawiki/2015/0/01/Standar-2-form-2-pic.png)
DNA ligation&Transformation
WangJie Liu and JunJie Xu
![](https://static.igem.org/mediawiki/2015/4/4b/Standar-3-form.png)
Successfully align the sequencing results with the objective sequences:
Colony PCR
WangJie Liu and JunJie Xu
![](https://static.igem.org/mediawiki/2015/f/fd/Standar-4-form.png)
![](https://static.igem.org/mediawiki/2015/c/c5/Standar-4-form-1-pic.png)
![](https://static.igem.org/mediawiki/2015/1/1f/Standar-4-form-2-pic.png)
![](https://static.igem.org/mediawiki/2015/9/90/Standar-4-form-3-pic.png)
![](https://static.igem.org/mediawiki/2015/5/56/Standar-4-form-4-pic.png)
![](https://static.igem.org/mediawiki/2015/6/66/Standar-4-form-5-pic.png)
Extract the plasmid,Conserving.
Week 7 8.10-8.16
PCR:
More details, protocol
WangJie Liu
![](https://static.igem.org/mediawiki/2015/3/34/Standar-5-form.png)
Restriction Enzyme Digestion
WangJie Liu
![](https://static.igem.org/mediawiki/2015/f/f9/Standar-6-form.png)
![](https://static.igem.org/mediawiki/2015/5/5b/Standar-5-form-1-pic.png)
DNA ligation&Transformation
WangJie Liu
![](https://static.igem.org/mediawiki/2015/9/98/Standar-7-form.png)
Colony PCR
Successfully align the sequencing results with the objective sequences:
WangJie Liu
![](https://static.igem.org/mediawiki/2015/2/2d/Standar-8-form.png)
Extract the plasmid,Conserving.
![](https://static.igem.org/mediawiki/2015/a/a7/S-l-W8P1.png)
![](https://static.igem.org/mediawiki/2015/1/1d/S-l-W8P2.png)
![](https://static.igem.org/mediawiki/2015/f/fc/S-l-W8P3.png)
![](https://static.igem.org/mediawiki/2015/a/a1/S-l-W8P4.png)
Week 9 8.24-8.30
![](https://static.igem.org/mediawiki/2015/2/27/S-l-W9P1.png)
Week 10 8.31-9.6
Successfully align the sequencing results with the objective sequences
Standalization of BD-CRY2 and AD-CIB1 finished.
![](https://static.igem.org/mediawiki/2015/d/da/S-l-W10P1.png)
![](https://static.igem.org/mediawiki/2015/8/89/S-l-W10P2.png)
![](https://static.igem.org/mediawiki/2015/1/1b/S-l-W10P3.png)
![](https://static.igem.org/mediawiki/2015/4/4a/S-l-W10P4.png)
Successfully align the sequencing results with the objective sequences
Standalization of CRY2 and CIB1 finished.
Light Control
Week 1 6.29-7.5
Week 2 7.6-7.12
![](https://static.igem.org/mediawiki/2015/1/15/L-prt-W2P1.png)
![](https://static.igem.org/mediawiki/2015/1/19/L-prt-W2P2.png)
![](https://static.igem.org/mediawiki/2015/6/64/L-prt-W2P3.png)
![](https://static.igem.org/mediawiki/2015/4/41/L-prt-W2P4.png)
![](https://static.igem.org/mediawiki/2015/4/4f/L-prt-W2P5.png)
Successfully align the sequencing results with the objective sequences of Pgal1 and CYC_TT.
Extract the plasmid.
Conserving.
Week 3 7.13-7.19
Week 4 7.20-7.26
![](https://static.igem.org/mediawiki/2015/9/9b/L-prt-W4P1.png)
![](https://static.igem.org/mediawiki/2015/8/8d/L-prt-W4P2.png)
![](https://static.igem.org/mediawiki/2015/d/dd/L-prt-W4P3.png)
Week 5 7.27-8.2
Successfully align the sequencing results with the objective sequences
![](https://static.igem.org/mediawiki/2015/9/94/L-prt-W5P1.png)
![](https://static.igem.org/mediawiki/2015/3/3e/L-prt-W5P2.png)
![](https://static.igem.org/mediawiki/2015/7/71/L-prt-W5P3.png)
![](https://static.igem.org/mediawiki/2015/1/1d/L-prt-W5P4.png)
![](https://static.igem.org/mediawiki/2015/5/5b/L-prt-W5P5.png)
Digestion Test
Guozhao Wu
Week 6 8.3-8.9
![](https://static.igem.org/mediawiki/2015/8/8a/L-prt-W6P1.png)
![](https://static.igem.org/mediawiki/2015/f/fb/L-prt-W6P2.png)
![](https://static.igem.org/mediawiki/2015/8/8a/L-prt-W6P3.png)
Successfully align the sequencing results with the objective sequences
Pgal-ROX1-CYC_TT circuit was constructed.
Week 7 8.10-8.16
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
viscous parts
Week 1 6.29-7.5
Week 2 7.6-7.12
PCR:
More details, protocol
Wang Xi and JunJie Xu
![](https://static.igem.org/mediawiki/2015/0/0e/STMcfp-1-form.png)
![](https://static.igem.org/mediawiki/2015/c/c0/STMcfp-1-form-1-pic.png)
Restriction Enzyme Digestion
Wang Xi and JunJie Xu
![](https://static.igem.org/mediawiki/2015/6/67/STMcfp-2-form.png)
![](https://static.igem.org/mediawiki/2015/9/95/STMcfp-2-form-1-pic.png)
DNA ligation&Transformation
Wang Xi and JunJie Xu
![](https://static.igem.org/mediawiki/2015/f/f9/STMcfp-3-form.png)
Colony PCR
Successfully align the sequencing results with the objective sequences:
Wang Xi and JunJie Xu
![](https://static.igem.org/mediawiki/2015/b/b7/STMcfp-4-form.png)
![](https://static.igem.org/mediawiki/2015/7/71/STMcfp-4-form-1-pic.png)
![](https://static.igem.org/mediawiki/2015/b/bf/STMcfp-4-form-2-pic.png)
Extract the plasmid,Conserving
Week 3 7.13-7.19
PCR:
More details, protocol
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/b/be/STMcfp-5-form.png)
![](https://static.igem.org/mediawiki/2015/a/a0/STMcfp-5-form-1-pic.png)
Restriction Enzyme Digestion
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/6/64/STMcfp-6-form.png)
DNA ligation&Transformation
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/0/07/STMcfp-7-form.png)
Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:
![](https://static.igem.org/mediawiki/2015/c/c3/STMcfp-8-form.png)
![](https://static.igem.org/mediawiki/2015/9/93/STMcfp-8-form-1-pic.png)
Extract the plasmid,Conserving.
Week 4 7.20-7.26
PCR:
More details, protocol
Wang Xi and JunJie Xu
![](https://static.igem.org/mediawiki/2015/c/ce/STMcfp-9-form.png)
Restriction Enzyme Digestion
Wang Xi and JunJie Xu
![](https://static.igem.org/mediawiki/2015/c/cc/STMcfp-10-form.png)
DNA ligation&Transformation
Wang Xi and ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/e/ec/STMcfp-11-form.png)
Colony PCR
Wang Xi and JunJie Xu
Successfully align the sequencing results with the objective sequences:
![](https://static.igem.org/mediawiki/2015/8/83/STMcfp-12-form.png)
![](https://static.igem.org/mediawiki/2015/f/f2/STMcfp-12-form-1-pic.png)
Extract the plasmid,Conserving.
Tramsformation to yeast
Lei Yin
![](https://static.igem.org/mediawiki/2015/0/0d/STMcfp-13-form.png)
Genome extract and PCR test
Lei Yin
![](https://static.igem.org/mediawiki/2015/c/c8/STMcfp-14-form.png)
Positive strain conservation
Week 5 7.27-8.2
PCR:
More details, protocol
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/4/46/STMcfp-15-form.png)
Restriction Enzyme Digestion
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/d/d1/STMcfp-16-form.png)
DNA ligation&Transformation
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/2/20/STMcfp-17-form.png)
Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:
![](https://static.igem.org/mediawiki/2015/6/63/STMcfp-18-form.png)
![](https://static.igem.org/mediawiki/2015/1/17/STMcfp-18-form-1-pic.png)
![](https://static.igem.org/mediawiki/2015/0/0a/STMcfp-18-form-2-pic.png)
![](https://static.igem.org/mediawiki/2015/0/0d/STMcfp-18-form-3-pic.png)
![](https://static.igem.org/mediawiki/2015/a/a0/STMcfp-18-form-4-pic.png)
Extract the plasmid,Conserving.
Trasnsform to yeast
![](https://static.igem.org/mediawiki/2015/a/a4/STMcfp-19-form.png)
Genome extract and PCR test
Lei Yin
![](https://static.igem.org/mediawiki/2015/c/c2/STMcfp-20-form.png)
Positive strain conservation
Week 6 8.3-8.9
PCR:
More details, protocol
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/9/99/STMcfp-21-form.png)
Restriction Enzyme Digestion
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/5/5d/STMcfp-22-form.png)
DNA ligation&Transformation
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/a/a9/STMcfp-23-form.png)
Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:
![](https://static.igem.org/mediawiki/2015/e/e9/STMcfp-24-form.png)
![](https://static.igem.org/mediawiki/2015/a/ad/STMcfp-24-form-1-pic.png)
![](https://static.igem.org/mediawiki/2015/a/a8/STMcfp-24-form-2-pic.png)
Extract the plasmid,Conserving.
Trasnsform to yeast
Lei Yin
![](https://static.igem.org/mediawiki/2015/d/d4/STMcfp-25-form.png)
Genome extract and PCR test
Lei Yin
![](https://static.igem.org/mediawiki/2015/2/21/STMcfp-26-form.png)
Positive strain conservation
Week 7 8.10-8.16
SDS-PAGE
Lei Yin
![](https://static.igem.org/mediawiki/2015/6/65/STMcfp-27-form.png)
Immunoflourecense
ZhangYu Cheng
![](https://static.igem.org/mediawiki/2015/a/ac/STMcfp-28-form.png)
Verification of the secreting Mcfp-3 (Coomassie blue staining)
ZhangYu Cheng
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
Improvement
Week 1 6.29-7.5
Week 2 7.6-7.12
Week 3 7.13-7.19
![](https://static.igem.org/mediawiki/2015/3/3f/I-W3P1.png)
![](https://static.igem.org/mediawiki/2015/3/39/I-W3P2.png)
Week 4 7.20-7.26
Week 5 7.27-8.2
![](https://static.igem.org/mediawiki/2015/4/49/I-W5P1.png)
![](https://static.igem.org/mediawiki/2015/d/dd/I-W5P2.png)
![](https://static.igem.org/mediawiki/2015/e/e3/I-W5P3.png)
![](https://static.igem.org/mediawiki/2015/3/33/I-W5P4.png)
![](https://static.igem.org/mediawiki/2015/6/6d/I-W5P5.png)
Week 6 8.3-8.9
![](https://static.igem.org/mediawiki/2015/e/ee/I-W6P1.png)
![](https://static.igem.org/mediawiki/2015/0/02/I-W6P2.png)
![](https://static.igem.org/mediawiki/2015/1/17/I-W6P3.png)
![](https://static.igem.org/mediawiki/2015/d/df/I-W6P4.png)
Week 7 8.10-8.16
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
Consolidation
Week 1 6.29-7.5
Week 2 7.6-7.12
Week 3 7.13-7.19
Week 4 7.20-7.26
Week 5 7.27-8.2
Week 6 8.3-8.9
Week 7 8.10-8.16
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
![](https://static.igem.org/mediawiki/2015/6/67/STYZ1.png)
![](https://static.igem.org/mediawiki/2015/d/d4/STYZ2.png)
![](https://static.igem.org/mediawiki/2015/8/82/STYZ3.png)
![](https://static.igem.org/mediawiki/2015/8/88/STYZ4.png)