iGEM Kent 2015
Notebook
Contents
Week 1
Week 2
Week 1
Day 1 Wet lab 22/06/15
Made LB plates
Made LB broth
TIPS autoclaved
Day 2 Wet lab 23/06/15
Set up Top10 overnight, VS45 overnight, US348 overnight
Day 3 Wet lab 24/06/15
Filter sterilise the buffer
250ml no antibiotic broth, put top10 cells in
Incubate until optical density is 0.6
Miniprep PSB1C3 plasmid
Day 4 Wet lab 25/06/15
Transformation
Prepare SBC media
Digest of PSB1c3 from MS348
Day 5 Wet lab 26/06/15
Calculated competent cell efficiency
Agarose gel formation
Gel electrophoresis
Week 2
Day 6 Wet lab 29/06/15
Transforming linear PSBC13