Team:UFMG Brazil/Results
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Project
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Lab Work
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Modeling
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Practices
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Synenergene
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Team
Results
DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in Leishmania host (Figure 1).
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Figure 1 - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.
The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. E. coli strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).
![](https://static.igem.org/mediawiki/2015/8/8f/UFMG-Brazil-leish-final.gif)