Team:Groningen/Notebook/Ligation th22
Blue Bio Energy
Ligation with thrC complete with inserts th22
Ligation of thrC complete plasmid with tasA, bslA or slrR
Extra integration of DNA in thrC locus
Ligated products
Ligation
00:00, 6 September 2015 - 00:00, 6 September 2015
The ligation of the thrC with inserts included ligating thrC with the Psalt + tasA and with Psalt + bslA. Finally slrR was also ligated in the thrC backbone. The ligations were incubated for 1 hour at room temperature.