Team:Kent/Experiments


iGEM Kent 2015

Experiments & Protocols

Contents

Competent Cells
Transformation Protocol
Miniprep

Competent Cells
Overview
Materials
Procedure
References
Transformation Protocol
Overview
Materials
Procedure
References
Miniprep
Overview

  • The Miniprep is for purification of molecular biology grade plasmid DNA
  • This provides a rapid method to purify plasmid DNA using silica membrane column

    • Materials
    Procedure

  • Add the provided RNase A solution to Buffer P1.
  • Mix the solution and store at 2–8°C
  • Add ethanol (96–100%) to Buffer PE before use
      • Pellet 1–5 ml bacterial overnight culture by centrifugation at >8000 rpm (6800 x g) for 3 min at room temperature (15–25°C).

    • References