Template:Team:Groningen/CONTENT/EXPERIMENTS/Ligation ferritin in pSB1C3 (fr3)
18:30, 31 August 2015 - 20:00, 10 August 2015
Digestion products were loaded on 1% agarose gel to purify.
Sample:
20 µL DNA (ferritin).
4 µL 6x buffer.
Ladder:
2 µL Thermo Scientific GeneRuler 1kb DNA ladder, ready to use.
Gel was run on 100V for 30 minutes. Gel results show a band in sample 1 of 1500bp and in sample 2 a band of 2000bp. This corresponds to the size of ferritin and pSB1C3. Gel was cut and dissolved in 2:1 binding buffer of the PCR purification kit and purified according to protocol. Samples were stored as: Ferritin dig (7,4ng/µL, Nanodrop value) and: pSB1C3 dig (6,2ng/µL, Nanodrop value).