Team:Groningen/Notebook/tasA Ligation t22
Blue Bio Energy
tasA K823023 Ligation (t22)
In this experiment tasA was ligated with the K823023 backbone to make transformation possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The K823023 backbone was ligated with tasA.
Ligation
00:00, 3 June 2015 - 00:00, 3 June 2015
For the ligation of tasA in BBa_K823023, the following components were used.
Component
Amount
Vector DNA (K823023)
2 µL
Insert DNA (tasA)
2 µL
10x buffer
2 µL
T4 ligase
1 µL
\( \mathrm{H_2O}\)
13 µL
Components used for ligation.
The ligation mixture was left 2 hours at room temperature.