Project Results
Here you can describe the results of your project and your future plans.
What should this page contain?
- Clearly and objectively describe the results of your work.
- Future plans for the project
- Considerations for replicating the experiments
TdT Variants
Sequences for the three different forms of TdT were designed and ordered. We successfully cloned each of these sequences into the biobrick vector pSB1C3 and the expression vector pET28. Samples were sent out for sequencing to confirm that the proper sequences were present in the plasmids. The biobrick plasmids were sent to igem while the pET28 vectors were transformed into the Rosetta cell line. Unfortunately this is as far as we got with this portion of the project. At this point, we only need to purify the proteins from the Rosetta cells and test them on oligonucleotides to make sure they function properly. From there, we would compare their efficiency with commercially available TdT using next gen sequencing technology.
Glass Slides
To test whether or not we had DNA on our glass slides we treated them with ethidium bromide (EtBr). Since EtBr is known to attach to DNA strands, any glass slides with DNA on them should also have EtBr. Exposing these slides to ultraviolet light would make the EtBr fluoresce allowing us to determine which slides had DNA present. Figure 1 shows that EtBr localized in the regions where DNA was added to the slides. On each slide, the number of oranges circles corresponds to the number and location of the regions DNA was added to. This is strong evidence that DNA was present on these slides. Figure 2 shows 4 different glass slides that underwent a wash step similar to the ones that would be done by the loomino device. The top two slides were negative controls and demonstrate that no residual EtBr was present in the absence of DNA bonded to the glass. The bottom two slides show slight orange fluorescence highlighted by the black boxes superimposed on the image. This indicates that there is at least some DNA present on these slides after the wash. It is unclear whether or not the DNA washed off or just the EtBr.
Project Achievements
You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.