Template:Team:Groningen/CONTENT/LOGBOOK/tasA Ligation pSB1C3 t41
tasA ligation pSB1C3 (t41)
The ligation of tasA in pSB1C3 to make the transformation in E. coli possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
tasA was ligated into pSB1C3.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 8 July 2015 - 00:00, 8 July 2015
Afterwards tasA was ligated in pSB1C3.
Component
Amount
tasA DNA
10 µL
pSB1C3 DNA
5 µL
T4 ligase
0.5 µL
T4 ligase buffer
2 µL
\( \mathrm{H_2O}\)
2.5 µL
Components used for ligation.
The sample was ligated at room temperature for 2 hours.