Team:Chalmers-Gothenburg/BioBricks

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• HOME
• TEAM
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• PROJECT
  • Theory
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• ACHIEVEMENTS
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• MODELING
  • Detection
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• NOTEBOOK
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• SAFETY
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• THANKS
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  • Acknowledgements

BioBricks

1. BBa_K1603000: STE2MAM2

Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.

2. BBa_K1603001: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. BBa_K1603002: pTPI1

Promoter to TPI1.

4. BBa_K1603003: RecA

Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.