Template:Team:Groningen/CONTENT/LOGBOOK/tasA Digestion Psalt t51
tasA Digestion Psalt (t51)
The salt promoter was digested to make ligation with tasA in the K823023 possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation of the salt promoter was performed.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 23 July 2015 - 00:00, 23 July 2015
For the ligation of Psalt, tasA and BBa_K823023, first the salt promoter (Psalt) had to be digested using EcoRI and SpeI as restriction enzymes. tasA and BBA_K823023 had been digested before already.
Component
Amount
DNA (Psalt)
10 µL
EcoRI
1 µL
SpeI
1 µL
10x buffer (2.1)
2 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion salt promoter.
The sample was digested for one hour at 37 °C.