Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Ligation (t53)

00:00, 23 July 2015 - 00:00, 23 July 2015

The subsequent ligation was performed with the folowing components.

Component

Amount

DNA 1 (Psalt)

5 µL

DNA 2 (tasA)

5 µL

DNA 3 (K823023)

5 µL

T4 ligase

1 µL

10x buffer

2 µL

\( \mathrm{H_2O}\)

2 µL

Components ligation Psalt, tasA and backbone.

The sample was ligated at room temperature for 2 hours.

Harm Ruesink