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week number 27

▼2015-07-01 11. In vitro transcription of the c-di GMP Spinach2 Variations

Aim: Synthesis of c-di GMP Aptamer Spinach2 RNA that will be used in the DFHBI Assay performed in the Tecan Safire 2

Procedure:

• Follow steps as described in protocol 7 and 8 for the elution of the RNA

Results: The UV shadowing of the denaturing polyacrylamide gel electrophoresis on a fluorescent TLC shows that we were able to transcribe both RNAs.