Template:Team:Groningen/CONTENT/LOGBOOK/tasA Sequencing t59
tasA Sequencing (t59)
The tasA DNA was send for sequencing to check if the sequence was correct.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
Sequencing showed that the tasA (T2) sample is correct.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/None">None</a>
00:00, 30 July 2015 - 00:00, 30 July 2015
The transformation of 29 July was checked. No colonies were visible on the LB+cm plate. T2 was send to Macrogen for sequencing.
Component
Amount
1/20 primer (5 pmol/µL)
5 µL
plasmid DNA (T2)
5 µL
Primer
Code
prefix primer (forward)
17C4ZAD658
suffix primer (reverse)
17C4ZAD659
The results from Macrogen showed that T2 has the right sequence.